| Project/Area Number |
16K14841
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Crop production science
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| Research Institution | University of Tsukuba (2017)
National Agriculture and Food Research Organization (2016) |
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Principal Investigator |
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| Research Collaborator |
MUSTAFA Ghazala
YASMEEN Farhat
HOSSAIN Zahed
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ストレス応答反応 / ナノ粒子 / ダイズ / 湿害 / プロテオミクス / プロテオーム / 植物 / ストレス |
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| Outline of Final Research Achievements |
To investigate the possible interactions between nanoparticles (NPs) and crops under flooding stress, the label-free proteomic technique was used. Alumina-NPs under flooding stress enhanced soybean growth compared to ZnO-NPs and Ag-NPs. Alumina-NPs-responsive proteins were related to protein synthesis/degradation, glycolysis, and lipid metabolism. Alumina-NPs promoted the growth of soybean under flooding stress by regulating energy metabolism and cell death. Mitochondrion was the target organelle of Alumina-NPs. Mitochondrial proteomic analysis revealed that the abundance of voltage-dependent anion channel protein was increased in soybeans with Alumina-NPs. Therefore, Alumina-NPs of various sizes affect mitochondrial proteins under flooding stress by regulating membrane permeability and tricarboxylic acid cycle activity. Furthermore, the energy regulation and membrane permeability might be the principal component for NPs interaction with soybean.
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