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Development of technology for leaf color-alteration by the control of gene expression using tissue layer- or floral differentiation period-specific promoter

Research Project

Project/Area Number 16K14849
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Horticultural science
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

suzuki sakae  東京農工大学, (連合)農学研究科(研究院), 講師 (80397017)

Research Collaborator ITO Risa  
EBE Yuriko  
KOZIMA Saeka  
SUGANOMATA Masumi  
YAMAMOTO Kaho  
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords葉色改変 / 組織特異的 / アントシアニン / カロテノイド / 花芽分化 / キメラ / 組織特異的プロモーター / RNAi / 葉脈 / フラボノイド / 園芸学 / 植物分子育種 / 植物色素
Outline of Final Research Achievements

In the present study, we try to develop a technology for leaf color-alteration by the control of gene expression using tissue layer- or floral differentiation period-specific promoter.
In the Tobacco plants, when the vectors of a combination with AtPIP2;1 or AtIAMT1 promoter and Atpap1 or NtPDS-RNAi gene were introduced, the color of leaf vein in transgenic plants was changed to red (Atpap1) or white (NtPDS-RNAi). Furthermore, the same results were observed in the plants of self-pollinated progenies. When the vectors of a combination with AtFT promoter and Atpap1 gene were introduced, in contrast, no color-alteration was observed in transgenic plants.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (5 results)

All 2018 2017 2016

All Presentation (5 results)

  • [Presentation] カロテノイド生合成遺伝子または花成関連遺伝子プロモーターを用いたタバコにおける花弁特異的な花色改変2018

    • Author(s)
      山本佳穂,倉持由衣,松崎光伯,鈴木栄
    • Organizer
      園芸学会平成30年度春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 機能性植物色素の蓄積を目的としたタバコおよびトマトへのフラボノイド・カロテノイド生合成関連遺伝子の導入2018

    • Author(s)
      菅野又ますみ,久永悠生,有江 力,鈴木栄
    • Organizer
      園芸学会平成30年度春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] トレニア由来 TfMYB1 遺伝子の過剰発現によるタバコの花色および葉色の改変2017

    • Author(s)
      伊藤莉沙,鈴木栄
    • Organizer
      園芸学会平成29年度秋季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 全身発現性プロモーターおよび緑色組織特異的プロモーターを用いた差分的RNAi法によるタバコの花色改変2016

    • Author(s)
      江部百合子,鈴木栄
    • Organizer
      園芸学会平成28年秋季大会
    • Place of Presentation
      名城大学天白キャンパス(名古屋市天白区塩釜口一丁目501番地)
    • Year and Date
      2016-09-10
    • Related Report
      2016 Research-status Report
  • [Presentation] ホトトギス属植物(Tricyrtis sp.)における再分化系確立と 花色改変に向けたカロテノイド生合成遺伝子の導入2016

    • Author(s)
      小嶋紗英香,中野優,鈴木栄
    • Organizer
      日本植物細胞分子生物学会
    • Place of Presentation
      信州大学繊維学部(長野県上田市常田 3-15-1)
    • Year and Date
      2016-09-01
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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