Analysis on proteins that are regulated transcription by PAT2 related parthenocapy of tomato.

• Project/Area Number: 16K14851
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Horticultural science
• Research Institution: Nagoya University
• Principal Investigator: Mori Hitoshi 名古屋大学, 生命農学研究科, 教授 (20220014)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 単為結果 / 単為結果性 / 転写因子 / zinc finger homeodomain

Outline of Final Research Achievements

Transcription factor PAT2 was found the causal gene of parthenocarpy in tomato. I identified target gene that was regulated by transcription factor PAT2 in order to analyze molecular mechanism of parthenocapy. Pat2 expression was decreased and GA3ox one, that is biosynthesis of active GA, was increased by auxin treatment using quantitative PCR in tomato ovary. Furthermore, Pat2 expression was decreased and GA3ox one was increased during developmental stage of ovary. These results indicated that PAT2 suppress GA3ox transcription and indicated that agricultural auxin treatment to tomato flower is this event.

Research Products

• [Journal Article] Genome of pitcher plant Cephalotus reveals genetic changes associated with carnivory (2017)
  • Author(s): Fukushima, K. et al.
  • Journal Title: Nat. Ecol. Evol. Volume: 1 Issue: 3 Pages: 0059-0059
  • DOI: 10.1038/s41559-016-0059
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An NADPH oxidase RBOH functions in rice roots during lysigenous aerenchyma formation under oxygen-deficient conditions. (2017)
  • Author(s): Yamauchi, T., Yoshioka, M., Fukazawa, A., Mori, H., Nishizawa, N.K., Tsutsumi, N., Yoshioka, H. and Nakazono, M.
  • Journal Title: The Plant Cell Volume: 印刷中 Issue: 4 Pages: 775-790
  • DOI: 10.1105/tpc.16.00976
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Loss of function at RAE2, a novel EPFL, is required for awnlessness in cultivated Asian rice. (2016)
  • Author(s): Bessho-Uehara K, Wang DR, Furuta T, Minami A, Nagai K, Gamuyao R, Asano A, Angeles-Shim RB, Shimizu Y, Ayano M, Komeda N, Doi K, Miura K, Toda Y, Kinoshita T, Okuda S, Higashiyama T, Nomoto M, Tada Y, Shinohara H, Matsubayashi Y, Greenberg A, Wu J, Yasui H, Yoshimura A, Mori H, McCouch SR, Ashikari M.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 113 Issue: 32 Pages: 8969-8974
  • DOI: 10.1073/pnas.1604849113
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Identification of a xylem sap germin-like protein and its expression under short-day and non-freezing low-temperature conditions in poplar root. (2016)
  • Author(s): Aohara, T., Mizuno, H., Kiyomichi, D., Abe, Y., Matsuki, K., Sagawa, K., Mori, H., Iwai, H., Furukawa, J. and Satoh, S.
  • Journal Title: Plant Biotech Volume: 33 Pages: 123-127
  • NAID: 130005164807
  • Peer Reviewed
• [Presentation] ラナンキュラス塊根の吸水発芽時に発現するα-galactosidaseタンパク質の発現解析 (2018)
  • Author(s): 森仁志, 村上恭子
  • Organizer: 園芸学会 平成30年度春季大会