Characterization of low pH and aluminum signal transduction based on chemical genetics
| Project/Area Number |
16K14870
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Gifu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
小郷 尚久 静岡県立大学, 薬学研究院, 講師 (20501307)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | アルミニウムシグナル / ケミカルスクリーニング / 酸性土壌耐性 / ALMT1 / STOP1 / in silicoドッキング / kinase阻害化合物ライブラリー / アルミニウム / シグナル伝達 / ホモロジーモデリング / 酸性土壌 / AtALMT1 / リンゴ酸分泌 / 化合物ライブラリー / シグナル伝達経路 / 植物栄養学 / ケミカルバイオロジー / アルミニウムストレス / 酸ストレス |
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| Outline of Final Research Achievements |
Low pH and Al-responsive AtALMT1 (Arabidopsis Al-activated malate transporter1) regulates malate secretion from roots. In this study, we identified some compounds inhibiting malate secretion and AtALMT1 gene expression in screening of small molecule library used in drug discovery research. Additionally, among these compounds, a kinase inhibitor for phospholipid signaling inhibited expression of some Al-responsive genes and STOP1 nuclear localization. Furthermore, in silico docking study indicated that the inhibitor may bind the Arabidopsis homologue of target protein in human. These results strongly suggested that phospholipid signaling play an important role in Al signaling.
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Report
(3 results)
Research Products
(3 results)
