Action mechanism and physiological function of coproporphyrins, novel bacterial growth factors

• Project/Area Number: 16K14876
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Hokkaido University
• Principal Investigator: Shigetomi Kengo 北海道大学, 農学研究院, 講師 (20547202)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: Leucobacter / Growth factor / Bacteria / Firmicutes / Actinobacteria / Coproporphyrin / coproporphyrin / zincphyrin / Actimobacteria / Proteobacteria / Heme biosynthesis / Heme / Leukobacter / Sphingopyxis / Heme synthesis / Symbiosis / Zincphyrin / 細菌 / 共生

Outline of Final Research Achievements

In this research, the action mechanism and physiological effectivities of coproporphyrins, novel bacterial growth factors were investigated. The results revealed that coproporphyrins function as the exogenous biosynthetic intermediate in the incomplete heme biosynthesis of Leucobacter sp. strain ASN212. Moreover, the growth factors were proved to stimulate a part of strains belonging to Firmicutes and gamma-Proteobacteria in bacterial flora.

Academic Significance and Societal Importance of the Research Achievements

実験室において培養できない微生物（難培養微生物）は環境微生物全体の99%以上と見積もられ、それらの中には人類に有用なものが存在すると推定される。難培養微生物を培養可能とするため、我々は微生物間でやりとりされる増殖因子としてコプロポルフィリン類を見出した。本課題では、それらがどのような機構で増殖を刺激し、どの程度その活性に普遍性があるのかを検証した。得られた成果は、難培養微生物の培養研究と技術開発において将来に大きく寄与するものである。

Research Products

• [Journal Article] 難培養放線菌Leucobacter sp. ASN212株が要求する増殖因子の探索 難培養微生物を培養可能にする新たな増殖因子の発見 (2019)
  • Author(s): 高井 亮吾, 重冨 顕吾, 生方 信
  • Journal Title: 化学と生物 Volume: 57 Pages: 143-145
  • NAID: 130007800447
  • Peer Reviewed
• [Journal Article] Growth Mechanism of Uncultured Actinobacterial Strain Leucobacter sp. ASN212 by Zinc Coproporphyrin (2017)
  • Author(s): Ryogo Takai, Kengo Shigetomi, Yoichi Kamagata, and Makoto Ubukata*
  • Journal Title: Heterocycles Volume: 95 Issue: 1 Pages: 145-151
  • DOI: 10.3987/com-16-s(s)31
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Coproporphyrins, novel interphylum growth factors released by Sphingopyxis sp., enable laboratory cultivation of previously uncultured Leucobacter sp. (2019)
  • Author(s): Ryogo Takai, Nazrul Islam Bhuiyan, Kengo Shigetomi, Shinya Mitsuhashi, Yoichi Kamagata, Makoto Ubukata
  • Organizer: ACS Spring 2019 National Meeting
  • Int'l Joint Research
• [Presentation] Leucobacter komagatae strain ASN212, a previously uncultured actinobacterium, require Coproporphyrin III as its growth factor (2018)
  • Author(s): Takai, R., Bhuiyan M. I. N., Shigetomi, K. Kamagata, Y. & Ubukata, M
  • Organizer: The International Conference on Beneficial Microbes (ICOBM 2018)
  • Int'l Joint Research
• [Presentation] Analysis of mechamism of Zinc coproporphyrins, novel growth factors for previously uncultured Leucobacter sp. ASN212 strain (2017)
  • Author(s): 髙井 亮吾, Mohammad Nazrul Bhuiyan, 重冨 顕吾, 鎌形 洋一, 生方 信
  • Organizer: 日本農芸化学会2017年大会
  • Place of Presentation: 京都女子大学（京都府・京都市）
  • Year and Date: 2017-03-17
• [Presentation] Leucobacter 属 A N212 株 に対する coporphyrin 金属錯体の増殖活性評価 (2016)
  • Author(s): 髙井 亮吾, Mohammad Nazrul Bhuiyan, 重冨 顕吾, 鎌形 洋一, 生方 信
  • Organizer: 平成28年度日本農芸化学会北海道支部第1回講演会
  • Place of Presentation: ホテル函館ロイヤル（北海道・函館市）
  • Year and Date: 2016-08-06