| Project/Area Number |
16K14878
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
橋本 義輝 筑波大学, 生命環境系, 准教授 (00323254)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 微生物 |
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| Outline of Final Research Achievements |
Using Streptomyces avermitilis strain whose whole genome sequence has been completely determined, we searched for proteins that are formed inducibly by the addition of each of various inducers, and identified the gene encoding one of the resultant inducible proteins. The transcriptional start site for the gene was determined by the primer extension analysis with RNA extracted from S. avermitilis cells that were grown in the medium in the presence of the inducer. Around the transcriptional start site, a possible promoter sequence and Shine-Dalgarno sequence were found, respectively. We constructed the disruptant mutant lacking the adjacent region including the gene. By the deletion analysis using the disruptant mutant as a host, we identified the regulatory gene responsible for the inducible formation of the gene in the presence of the inducer.
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