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Development of method using synthetic biology to design artificial biosynthetic pathway.

Research Project

Project/Area Number 16K14880
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied microbiology
Research InstitutionThe University of Tokyo

Principal Investigator

Katsuyama Yohei  東京大学, 大学院農学生命科学研究科(農学部), 准教授 (50646437)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsengineering / biosynthesis / directed evolution / biosensor / バイオセンサー / 指向性進化 / 酵素 / 生合成 / 合成生物学 / 微生物生産 / 進化工学
Outline of Final Research Achievements

We constructed screening system to modify the ligand specificity of XylS which is a transcriptional activator which recognize m-toluic acid. Based on this system we carried six round of directed evolution and obtained XylS mutant which recognized p-toulic acid. This mutant has 5 mutations. Further analysis showed that three of them are important for p-toluic acid recognition. Saturated mutagenesis at these three sites resulted in XylS mutant which recognize p-toluic acid more selectively. By improving this screening system, we would like to develop a system to design artifical biosynthetic pathway. We also attempted to construct screening system to engineer stilbene synthase by using FdeR and QdoR. However, these enzymes do not have appropriate property for screening.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (2 results)

All 2018 2017

All Presentation (2 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] Modification of Ligand Specificity of XylS through Directed Evolution.2018

    • Author(s)
      Yohei Katsuyama, ○Yuki Ogawa, Kento Ueno and Yasuo Ohnishi
    • Organizer
      18th International Symposium on the Biology of Actinomycetes (International Convention Center JEJU)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 指向性進化による転写因子XylSのリガンド認識能の改変2017

    • Author(s)
      勝山 陽平、○小川 友希、上野 堅登、大西 康夫
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都女子大学 (京都)
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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