| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
We constructed screening system to modify the ligand specificity of XylS which is a transcriptional activator which recognize m-toluic acid. Based on this system we carried six round of directed evolution and obtained XylS mutant which recognized p-toulic acid. This mutant has 5 mutations. Further analysis showed that three of them are important for p-toluic acid recognition. Saturated mutagenesis at these three sites resulted in XylS mutant which recognize p-toluic acid more selectively. By improving this screening system, we would like to develop a system to design artifical biosynthetic pathway. We also attempted to construct screening system to engineer stilbene synthase by using FdeR and QdoR. However, these enzymes do not have appropriate property for screening.
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