| Project/Area Number |
16K14882
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Nagoya University |
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Principal Investigator |
Hemmi Hisashi 名古屋大学, 生命農学研究科, 准教授 (60302189)
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| Research Collaborator |
MOTOYAMA Kento 名古屋大学, 大学院生命農学研究科, 大学院生
KOKUBO Takuya 名古屋大学, 大学院生命農学研究科, 大学院生
YOSHIDA Ryo 名古屋大学, 大学院生命農学研究科, 大学院生
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | アーキア / 膜脂質 / イソプレノイド / 微生物生産 / メバロン酸経路 / バクテリア / 細胞膜 / 酵素 / 応用微生物 / 脂質 / 微生物 |
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| Outline of Final Research Achievements |
The structures of membrane lipids from archaea are different from those from other organisms, bacteria and eukaryotes. The difference causes the low permeability of membrane composed of archaeal membrane lipids to small molecules such as ions, which is considered to enable archaea to grow under extreme conditions. Thus, we have forced the bacterium Escherichia coli to produce archaeal membrane lipids in order to strengthen its cell membrane. In this study, we introduced a biosynthetic pathway of the precursors of archaeal membrane lipids in E. coli to increase their production levels. And, we elucidated the biosynthetic pathway of membrane lipids specific to a hyperthermophilic archaeon, which are expected to have greater effects on the strengthening of membrane, and constructed the production system of the lipids in E. coli.
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