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Analysis and in vitro reconstitution of biosilica layers that show acid resistance and selective permeability

Research Project

Project/Area Number 16K14888
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied microbiology
Research InstitutionHiroshima University

Principal Investigator

Ikeda Takeshi  広島大学, 先端物質科学研究科, 助教 (10505754)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords細菌 / 胞子 / ケイ素 / 応用微生物
Outline of Final Research Achievements

Bacillus cereus and its close relatives take up soluble silicate from the environment and accumulate it as insoluble silica in and around the spore coat layer. The silica layer serves as a protective coating against acid while showing permeability towards germinant molecules. To elucidate the mechanism underlying these dual functions, we extracted organic molecules from the silica layer. MS analysis revealed the presence of organic polymers containing multiple amino groups. A gene disruptant lacking these polymers formed a silica layer whose nanostructures were slightly different from those of the wild-type one. These results suggest that the polymers embedded in the silica layer might play an important role in the functions of the silica layer.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (4 results)

All 2018 2017 2016

All Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] 中温性グラム陽性細菌Bacillus cereusは胞子表面に無機固体であるシリカを形成し、その内部には長鎖ポリアミンが存在する2018

    • Author(s)
      池田 丈, 中川 美樹, 山本 光士郎, 廣田 隆一, 黒田 章夫
    • Organizer
      日本ポリアミン学会第9回年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Bacillus属細菌が形成するシリカ内部に含まれる長鎖ポリアミンの発見2017

    • Author(s)
      中川 美樹, 田中 達也, 池田 丈, 廣田 隆一, 黒田 章夫
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Discovery of long-chain polyamines (LCPAs) in a silica layer of the gram-positive, spore-forming bacterium Bacillus cereus2017

    • Author(s)
      Miki Nakagawa, Takeshi Ikeda, Tatsuya Tanaka, Kohjiro Yamamoto, Ryuichi Hirota, Akio Kuroda
    • Organizer
      BiominXIV/14th International Symposium on biomineralization
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Bacillus属細菌のシリカ蓄積メカニズムの解析2016

    • Author(s)
      池田 丈, 田中 達也, 中川 美樹, 本村 圭, 廣田 隆一, 黒田 章夫
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山国際会議場・ANAクラウンプラザホテル富山
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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