Analysis and in vitro reconstitution of biosilica layers that show acid resistance and selective permeability
Project/Area Number |
16K14888
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Applied microbiology
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Research Institution | Hiroshima University |
Principal Investigator |
Ikeda Takeshi 広島大学, 先端物質科学研究科, 助教 (10505754)
|
Project Period (FY) |
2016-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 細菌 / 胞子 / ケイ素 / 応用微生物 |
Outline of Final Research Achievements |
Bacillus cereus and its close relatives take up soluble silicate from the environment and accumulate it as insoluble silica in and around the spore coat layer. The silica layer serves as a protective coating against acid while showing permeability towards germinant molecules. To elucidate the mechanism underlying these dual functions, we extracted organic molecules from the silica layer. MS analysis revealed the presence of organic polymers containing multiple amino groups. A gene disruptant lacking these polymers formed a silica layer whose nanostructures were slightly different from those of the wild-type one. These results suggest that the polymers embedded in the silica layer might play an important role in the functions of the silica layer.
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Report
(3 results)
Research Products
(4 results)