| Project/Area Number |
16K14893
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Setsunan University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
橋本 渉 京都大学, 農学研究科, 教授 (30273519)
丸山 如江 摂南大学, 理工学部, 助教 (90397563)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 好アルカリ性細菌 / 原形質分離 / ATP合成機構 / プロトン勾配 / 細菌内膜動的構造 / プロトン駆動力 / F0F1ATPase / pHジャンプ / Aeromonas属細菌 / 内膜皺構造 / 細胞膜機能 / 廃グリセロール / アエロモナス属細菌 / 窒素固定細菌 / 有機酸生産 |
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| Outline of Final Research Achievements |
It has been postulated that alkalophilic bacteria synthesize ATP by the action of membrane-bound F0F1-ATPase, thereby utilizing proton (H+) chemical gradient formed across the cytoplasmic membrane as an energy source. However, in alkaline growth conditions, it seems difficult to accumulate H+ outside the membrane, since H+ is readily converted to H2O through the reaction with OH- present in external milieu at extremely high concentration. We found that the cells of Aeromonas sp. strain L, a Gram negative rod, repeat plasmolysis in accordance with cell growth (i.e. cell division) and postulated the possibility that the physicochemical structures induced during a contraction and expansion of cytoplasmic membrane will prepare place for H+ accumulation and form chemical gradient of H+ across the cell membrane. This physicochemical mechanism for the ATP synthesis will be expected to exclude the unclear hitherto explanation as to the ATP synthesis in alkalophilic bacteria.
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