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Development of a novel construction method of polyploid in yeast

Research Project

Project/Area Number 16K14894
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied microbiology
Research InstitutionSojo University

Principal Investigator

HARASHIMA Satoshi  崇城大学, 生物生命学部, 教授 (70116086)

Co-Investigator(Kenkyū-buntansha) 浴野 圭輔  崇城大学, 生物生命学部, 准教授 (30310030)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords酵母 / "超"高次倍数体 / “超”高次倍数体 / 接合型変異 / 育種学 / 応用微生物学 / 接合型 / バイオテクノロジー / 倍数性 / 応用微生物 / 細胞工学
Outline of Final Research Achievements

How ploidy in organisms is controlled is one of the important issues in modern biology. Industrial strains of budding yeast is well known to be polyploid is also interesting fact in biotechnology in connection with useful characteristics. However, research regarding physiological significance of ploidy is not advanced due to unavailable technology to construct polyploid at will. In this study, we have developed a novel breeding technology to construct a series of polyploid strain by using a specific mutation of mating type αin budding yeast. We have created hexaploid, octoploid and decaploid using this technique. We are now challenging whether more higher polyploid strain could be created.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (20 results)

All 2017 2016

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 5 results) Presentation (10 results) (of which Invited: 1 results) Book (2 results)

  • [Journal Article] Molecular breeding of Saccharomyces cerevisiae with high RNA content by harnessing essential ribosomal RNA transcription regulator2017

    • Author(s)
      Yu Sasano , Takahiro Kariya , Shogo Usugi , Minetaka Sugiyama and Satoshi Harashima
    • Journal Title

      AMB Express

      Volume: 7 Issue: 1

    • DOI

      10.1186/s13568-017-0330-4

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Simultaneous Hydrolysis and Fermentation of Sweet Sorghum Varieties (FS501 and KCS105) into Bioethanol Using Saccharomyces steineri -A Kinetics Study2017

    • Author(s)
      Hanifrahmawan Sudibyo, Jasman Himawan Tri Bayu Murti Petrus, Agus Prasetya, Donny Widianto, Chusnul Hidayat, Irfan Dwidya Prijambada, Satoshi Harashima
    • Journal Title

      Engineering Journal

      Volume: 21 Issue: 7 Pages: 105-121

    • DOI

      10.4186/ej.2017.21.7.105

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] CRISPR-PCS Protocol for Chromosome Splitting and Splitting Event Detection in Saccharomyces cerevisiae2017

    • Author(s)
      Yu Sasano and Satoshi Harashima
    • Journal Title

      Bio-protocol

      Volume: 7 Issue: 10

    • DOI

      10.21769/bioprotoc.2306

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cellular mechanisms contributing to multiple stress tolerance in Saccharomyces cerevisiae strains with potential use in high-temperature ethanol fermentation.2016

    • Author(s)
      Yasin Kitichantaropas; Chuenchit Boonchird; Minetaka Sugiyama; Yoshinobu Kaneko; Satoshi Harashima; Choowong Auesukaree, Ph.D
    • Journal Title

      AMB Express

      Volume: 6 Issue: 1 Pages: 1-14

    • DOI

      10.1186/s13568-016-0285-x

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] CRISPR-PCS: a powerful new approach to inducing multiple chromosome splitting in Saccharomyces cerevisiae2016

    • Author(s)
      Yu Sasano, Koki Nagasawa, Saeed Kaboli, Minetaka Sugiyama, Satoshi Harashima
    • Journal Title

      Scientific Reports.

      Volume: 6 Issue: 1 Pages: 1-11

    • DOI

      10.1038/srep30278

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Overexpression of ESBP6 improves lactic acid resistance and production in Saccharomyces cerevisiae.2016

    • Author(s)
      Sugiyama M, Akase SP, Nakanishi R, Kaneko Y, Harashima S
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 印刷中 Issue: 4 Pages: 415-420

    • DOI

      10.1016/j.jbiosc.2016.03.010

    • NAID

      40020966107

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Core regulatory components of the PHO pathway are conserved in the methylotrophic yeast Hansenula polymorpha.2016

    • Author(s)
      Zhou Y, Yuikawa N, Nakatsuka H, Maekawa H, Harashima S, Nakanishi Y, Kaneko Y.
    • Journal Title

      Curr Genet.

      Volume: 62 Issue: 3 Pages: 595-605

    • DOI

      10.1007/s00294-016-0565-7

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Improved stress resistance and ethanol production by segmental haploidization of the diploid genome in Saccharomyces cerevisiae2016

    • Author(s)
      Kaboli S, Miyamoto T, Sunada K, Sasano Y, Sugiyama M, Harashima S
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 未定 Issue: 6 Pages: 638-644

    • DOI

      10.1016/j.jbiosc.2015.10.012

    • NAID

      40020873418

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] 出芽酵母における“超”高次倍数体育種技術の開発2017

    • Author(s)
      糸数帆高,松本拓己,浴野圭輔,西澤正文,原島 俊
    • Organizer
      第24回 日本生物工学会九州支部 沖縄大会(2017)
    • Related Report
      2017 Annual Research Report
  • [Presentation] バガス由来阻害物耐性出芽酵母変異株の分離と遺伝解析2017

    • Author(s)
      谷 龍典,門畑凌太,村上亮輔,藤本昌希,平川万里,浴野圭輔,原島 俊
    • Organizer
      第24回 日本生物工学会九州支部 沖縄大会(2017)
    • Related Report
      2017 Annual Research Report
  • [Presentation] Bacillus subtilis NBRC13719由来抗菌ペプチドの解析2017

    • Author(s)
      前山 諒,岡 拓二,原島 俊,浴野圭輔
    • Organizer
      第24回 日本生物工学会九州支部 沖縄大会(2017)
    • Related Report
      2017 Annual Research Report
  • [Presentation] CRISPR-PCS法を基盤とした酵母染色体の多様な操作技術の開発2016

    • Author(s)
      笹野 佑, 長澤 宏器, 木村 駿太, Kaboli Saeed, 中井 大志, 村山 亮太, 杉山 峰崇, 原島 俊
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山国際会議場ANAクラウンプラザホテル富山(富山県富山市)
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report
  • [Presentation] 高温耐性酵母 Ogataea polymorpha による乳酸生産2016

    • Author(s)
      杉山 峰崇, 加藤 謙斗, 深谷 健, 橋本 晋弥, 笹野 佑, 金子 嘉信, 原島 俊
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山国際会議場ANAクラウンプラザ富山(富山県富山市)
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report
  • [Presentation] - A new challenge to deciphering genome function and breeding -2016

    • Author(s)
      Satoshi Harashima, Minetaka Sugiyama, Yu Sasano
    • Organizer
      The 14th International Congress on Yeasts
    • Place of Presentation
      兵庫県立淡路夢舞台国際会議場(兵庫県淡路市)
    • Year and Date
      2016-09-11
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] CRISPR-PCS: a versatile chromosome manipulation technology in Saccharomyces cerevisiae2016

    • Author(s)
      Yu Sasano, Koki Nagasawa, Shunta Kimura, Saeed Kaboli, Taishi Nakai, Ryota Murayama, Minetaka Sugiyama, Satoshi Harashima
    • Organizer
      The 14th International Congress on Yeasts
    • Place of Presentation
      兵庫県立淡路夢舞台国際会議場(兵庫県淡路市)
    • Year and Date
      2016-09-11
    • Related Report
      2016 Research-status Report
  • [Presentation] Genetic engineering of the thermotolerant yeast Ogataea polymorpha for lactic acid production2016

    • Author(s)
      Kento Kato, Takeru Fukaya, Shinya Hashimoto, Yu Sasano, Yoshinobu Kaneko, Satoshi Harashima, Minetaka Sugiyama
    • Organizer
      The 14th International Congress on Yeasts
    • Place of Presentation
      兵庫県立淡路夢舞台国際会議場(兵庫県淡路市)
    • Year and Date
      2016-09-11
    • Related Report
      2016 Research-status Report
  • [Presentation] 出芽酵母において多様な染色体操作を可能にするCRISPR-PCS法の開発2016

    • Author(s)
      笹野 佑, 長澤宏器, 木村駿太, Kaboli Saeed, 中井大志, 村山亮太, 杉山峰崇, 原島 俊
    • Organizer
      酵母遺伝学フォーラム第49回研究報告会
    • Place of Presentation
      シーサイドホテル舞子ビラ(兵庫県神戸市)
    • Year and Date
      2016-09-09
    • Related Report
      2016 Research-status Report
  • [Presentation] Ogataea polymorphaによる高効率乳酸発酵2016

    • Author(s)
      杉山峰崇、加藤謙斗、深谷 健、橋本晋弥、笹野 佑、金子嘉信、原島 俊
    • Organizer
      酵母遺伝学フォーラム第49回研究報告会
    • Place of Presentation
      シーサイドホテル舞子ビラ(兵庫県神戸市)
    • Year and Date
      2016-09-09
    • Related Report
      2016 Research-status Report
  • [Book] リン酸シグナル伝達 リンの事典(大竹久夫著書)2017

    • Author(s)
      原島 俊
    • Total Pages
      9
    • Publisher
      朝倉書店
    • Related Report
      2017 Annual Research Report
  • [Book] 化学と生物「高温・有機酸ストレス耐性出芽酵母の育種と発酵生産への利用」2016

    • Author(s)
      杉山 峰崇, 笹野 佑, 鈴木 俊宏, 原島 俊
    • Publisher
      国際文献社
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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