| Project/Area Number |
16K14899
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TANOKURA Masaru 東京大学, 大学院農学生命科学研究科(農学部), 教授 (60136786)
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| Project Period (FY) |
2016-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | ミトコンドリア / 代謝動態 / 線虫 / 老化 / 寿命 |
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| Outline of Final Research Achievements |
Mitochondrion is an organelle for energy production by the TCA cycle and electron transfer system, and is also involved in the regulation of lifespan and organ aging such as age-related hearing loss and sarcopenia. In this study, we analyzed age-related changes in mitochondrial metabolites and the effect of metabolites that prolong the lifespan of C. elegans (nematode), which is a typical model organism of lifespan studies. Isolation of mitochondria from nematodes was established by the combination of multi-beads shocker and centrifugation. On the other hand, age-related changes of metabolites were examined by supercritical fluid extraction LC-MS/MS analysis. Finally, we focused on purine nucleotide metabolism based on previously reported data, and then obtained the results that purine nucleotides prolonged the life span of nematodes.
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