Does caffene function as rhizosphere signal?

• Project/Area Number: 16K14901
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Kyoto University
• Principal Investigator: Sugiyama Akifumi 京都大学, 生存圏研究所, 准教授 (20598601)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: カフェイン / 根圏微生物 / 根圏

Outline of Final Research Achievements

In this study transgenic tomato plants expressing genes involved in the caffeine synthesis was generated. These transgenic plants synthesized caffeine and secreted as well. The effect of caffeine to the rhizosphere microbes was analyzed. More than 20 strains were isolated from rhizosphere soils of coffee plants. No of these bacteria showed caffeine metabolizing activities. The effect of caffeine to these bacterial was tested in vitro. Caffeine synthesis and secretion were also analyzed using the seedlings of coffee plants. From the genome analysis and gene expression analysis several candidate genes involved in the caffeine secretion were isolated.

Research Products

• [Journal Article] 根圏微生物のダイズ持続的生産への活用 (2017)
  • Author(s): 杉山暁史
  • Journal Title: アグリバイオ Volume: 9 Pages: 28-31
  • Peer Reviewed
• [Journal Article] Caffeine fostering of mycoparasitic fungi against phytopathogens. (2016)
  • Author(s): Sugiyama, A., Sano, CM. Yazaki, K., Sano, H.
  • Journal Title: Plant signaling & behavior Volume: 11 Issue: 1 Pages: e1113362-e1113362
  • DOI: 10.1080/15592324.2015.1113362
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] コーヒーノキ根圏の微生物叢解析及びカフェイン代謝菌の探索 (2018)
  • Author(s): 川上智、矢崎一史、杉山暁史
  • Organizer: 日本農芸化学会2018年度大会
• [Presentation] コーヒーノキにおけるPurine Permeaseの発現及びカフェイン取り込み輸送解析 (2018)
  • Author(s): 掛川博文、士反伸和、永山秀佳、荻田信二郎、矢崎一史、杉山暁史
  • Organizer: 日本農芸化学会2018年度大会
• [Presentation] コーヒーノキからのカフェイン分泌と根圏での分解動態の解析 (2017)
  • Author(s): 川上智、永山 秀佳、荻田信二郎、矢崎一史、杉山暁史
  • Organizer: 日本農芸化学会 2017年度大会
  • Place of Presentation: 京都
  • Year and Date: 2017-03-17
• [Presentation] 根圏に分泌される植物化学物質の動態と機能 (2017)
  • Author(s): 杉山暁史
  • Organizer: 植物化学シンポジウム
  • Invited
• [Presentation] Analysis of caffeine secretion from intact Coffea plants and the cell cultures (2017)
  • Author(s): Hirofumi Kakegawa, Tomo Kawakami, Nobukazu Shitan, Shuka Nagayama, Shinjiro Ogita, Kazufumi Yazaki and Akifumi Sugiyama
  • Organizer: The 7th International Symposium for Sustainable Humanosphere (ISSH)
  • Int'l Joint Research
• [Presentation] コーヒーノキ根圈におけるカフェイン代謝菌の探索 (2017)
  • Author(s): 川上智、矢崎一史、杉山暁史
  • Organizer: 植物微生物研究会第 27 回研究交流会
• [Presentation] コーヒーノキ根圏へのカフェイン分泌と輸送体の探索 (2017)
  • Author(s): 掛川博文、士反伸和、永山秀佳、荻田信二郎、矢崎一史、杉山暁史
  • Organizer: 植物微生物研究会第 27 回研究交流会
• [Presentation] コーヒーノキからのカフェイン分泌と根圏での分解動態の解析 (2017)
  • Author(s): 川上智、永山秀佳、荻田信二郎、矢崎一史、杉山暁史
  • Organizer: 日本農芸化学会 2017年度大会
• [Presentation] コーヒーノキ根からのカフェイン分泌の解析 (2016)
  • Author(s): 川上 智、矢崎 一史、杉山暁史
  • Organizer: 植物微生物研究会
  • Place of Presentation: 仙台
  • Year and Date: 2016-09-07