Development a novel method to produce a single protein in yeast

Research Project

Project/Area Number	16K14906
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Applied biochemistry
Research Institution	Osaka City University
Principal Investigator	Yamaguchi Yoshihiro 大阪市立大学, 複合先端研究機構, 特任准教授(テニュアトラック) (00737009)
Project Period (FY)	2016-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000) Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	SPP system / MqsR / MazF / yeast / Yeast / Saccharomyces cerevisiae / 応用微生物 / バイオテクノロジー / バイオリアクター
Outline of Final Research Achievements	In this study, our goal is to develop SPP system in Saccharomyces cerevisiae. To archive it, I first construct two plasmids. One is to express sequence-specific endoribonuclease and another is to express target protein. We constructed the two plasmids to express MazF or MqsR and a target protein, respectively. We tried may conditions however we could not develop yeast SPP system.

Report

(3 results)