Construction of artificial blue light receptor for synthetic phototaxis of purple photosynthetic bacteria
| Project/Area Number |
16K14907
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
Azai Chihiro 立命館大学, 生命科学部, 助教 (70706564)
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| Research Collaborator |
SHIMOJI Mamiko
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 走光性 / 鞭毛モーター / LOV / CheA / ヒスチジンキナーゼ / 紅色光合成細菌 / 青色光受容体 |
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| Outline of Final Research Achievements |
Through the course of this project, we tried to construct a chimera of class I and II histidine kinases (HK) to construct a photoresponsive HK that enable us switch the rotation direction of bacterial flagellar motors by light irradiation. We designed an artificial blue-light receptor protein “YC”, which is a chimera of LOV-HK and CheA, based on homology in their atomic structures. Swarming assay of the YC-expressed E. coli showed acquired phototactic response by the light-dependent autophosphorylation activity of YC. The recombinant YC protein had a photoconvertable flavin cofactor that adducts to the conserved Cys residue of the LOV domain. This is the first successful report for functional fusion of the class I and II HKs.
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Report
(3 results)
Research Products
(3 results)
