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Construction of artificial blue light receptor for synthetic phototaxis of purple photosynthetic bacteria

Research Project

Project/Area Number 16K14907
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied biochemistry
Research InstitutionRitsumeikan University

Principal Investigator

Azai Chihiro  立命館大学, 生命科学部, 助教 (70706564)

Research Collaborator SHIMOJI Mamiko  
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords走光性 / 鞭毛モーター / LOV / CheA / ヒスチジンキナーゼ / 紅色光合成細菌 / 青色光受容体
Outline of Final Research Achievements

Through the course of this project, we tried to construct a chimera of class I and II histidine kinases (HK) to construct a photoresponsive HK that enable us switch the rotation direction of bacterial flagellar motors by light irradiation. We designed an artificial blue-light receptor protein “YC”, which is a chimera of LOV-HK and CheA, based on homology in their atomic structures. Swarming assay of the YC-expressed E. coli showed acquired phototactic response by the light-dependent autophosphorylation activity of YC. The recombinant YC protein had a photoconvertable flavin cofactor that adducts to the conserved Cys residue of the LOV domain. This is the first successful report for functional fusion of the class I and II HKs.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (3 results)

All 2018

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (2 results)

  • [Journal Article] Rapid C8-vinyl reduction of divinyl-chlorophyllide a by BciA from Rhodobacter capsulatus2018

    • Author(s)
      Azai Chihiro、Kobayashi Manami、Mizoguchi Tadashi、Tamiaki Hitoshi、Terauchi Kazuki、Tsukatani Yusuke
    • Journal Title

      Journal of Photochemistry and Photobiology A: Chemistry

      Volume: 353 Pages: 661-666

    • DOI

      10.1016/j.jphotochem.2017.09.010

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 異なるクラスのヒスチジンキナーゼを融合した人工青色光受容体タンパク質の作製2018

    • Author(s)
      下地 真美子,福原 友輔,笠原 賢洋,寺内 一姫,浅井 智広
    • Organizer
      第59回 日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 光応答性CheA タンパク質の設計:異なるクラスのヒスチジンキナーゼのキメラ化2018

    • Author(s)
      下地 真美子,福原 友輔,笠原 賢洋,寺内 一姫,浅井 智広
    • Organizer
      第12回日本ゲノム微生物学会年会
    • Related Report
      2017 Annual Research Report

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Published: 2016-04-21   Modified: 2019-03-29  

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