Elucidation and utilization of biosynthetic pathway not involving known isopentenyl diphosphate isomerase homologs
| Project/Area Number |
16K14911
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bioorganic chemistry
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| Research Institution | Niigata University |
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Principal Investigator |
Sato Tsutomu 新潟大学, 自然科学系, 准教授 (80334655)
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| Research Collaborator |
KUZUYAMA Tomohisa
UMENO Daisuke
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 生合成 / イソプレノイド / テルペノイド / プレニル鎖伸長酵素 |
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| Outline of Final Research Achievements |
Farnesyl diphosphate synthetase synthesized farnesyl diphosphate using only isopentenyl diphosphate as a substrate. This result suggested that it also has isopentenyl diphosphate isomerase activity. That is, it turned out that it is a bifunctional enzyme. Currently, we are conducting research aimed at elucidating the catalytic mechanism.
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| Academic Significance and Societal Importance of the Research Achievements |
新型酵素や二機能性酵素が関与するイソプレノイド生合成の新規基幹経路や新しい触媒機構の解明は、生物有機化学の基礎研究として重要なだけでなく、薬剤・香料・バイオ燃料などのバイオ生産や医薬開発などの応用に新しい展開をもたらすものと期待される。
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] Crystal structure and functional analysis of large-terpene synthase belonging to a newly found subclass2018
Author(s)
Masahiro Fujihashi, Tsutomu Sato, Yuma Tanaka, Daisuke Yamamoto, Tomoyuki Nishi, Daijiro Ueda, Mizuki Murakami, Yoko Yasuno, Ai Sekihara, Kazuma Fuku, Tetsuro Shinada and Kunio Miki,
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Journal Title
Chemical Science
Volume: 9
Issue: 15
Pages: 3754-3758
DOI
NAID
Related Report
Peer Reviewed / Open Access
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