Analysis of gene expression depending on energy status by epigenome regulation

• Project/Area Number: 16K14919
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Food science
• Research Institution: Tokyo University of Agriculture (2018); The University of Tokyo (2016-2017)
• Principal Investigator: INOUE Jun 東京農業大学, 応用生物科学部, 准教授 (70323962)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: エピゲノム / マイクロアレイ / エピゲノム制御 / 遺伝子発現

Outline of Final Research Achievements

The purpose of this study is to understand the mechanism of regulation of gene expression depending on energy status. Comprehensive gene expression analysis was performed using a microarray experiment to select the genes to be analyzed in this study. Then, I examined the histone modification of several selected genes and found that these genes could be divided into two groups.

Academic Significance and Societal Importance of the Research Achievements

エピゲノム制御は、発生やリプログラミング、がん等の疾患発症に関与することが明らかになりつつある。本研究は、劇的に遺伝子発現が変動することが知られている絶食・再摂食に着目し、生体のエネルギー状態の変動がおよぼす遺伝子発現の変化に、エピゲノム制御が関与するかどうかを明らかにすることを目指した。その結果、転写因子の活性化だけでは説明のつかない発現変動をうける遺伝子を見出した。これはエピゲノムレベルで生体内エネルギー制御を行っている可能性を示す結果であり、今後のさらなる解析により、制御の実体を明らかにすることで、生体のエネルギー代謝制御機構の理解につながることが期待される。

Research Products

• [Journal Article] Chrysin reduces the activity and protein level of mature forms of sterol regulatory element-binding proteins (2019)
  • Author(s): Iwase Masamori、Watanabe Kyoko、Shimizu Makoto、Suzuki Tsukasa、Yamamoto Yuji、Inoue Jun、Sato Ryuichiro
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: in press Issue: 9 Pages: 1-7
  • DOI: 10.1080/09168451.2019.1608806
  • Peer Reviewed
• [Journal Article] FGF21 Alleviates Hepatic Endoplasmic Reticulum Stress under Physiological Conditions (2018)
  • Author(s): MARUYAMA Ryuto、SHIMIZU Makoto、HASHIDUME Tsutomu、INOUE Jun、ITOH Nobuyuki、SATO Ryuichiro
  • Journal Title: Journal of Nutritional Science and Vitaminology Volume: 64 Issue: 3 Pages: 200-208
  • DOI: 10.3177/jnsv.64.200
  • NAID: 130007413207
  • ISSN: 0301-4800, 1881-7742
  • Peer Reviewed
• [Journal Article] Isoxanthohumol stimulates ubiquitin-proteasome-dependent degradation of precursor forms of sterol regulatory element-binding proteins (2018)
  • Author(s): Inoue Jun、Miyata Shingo、Shimizu Makoto、Sato Ryuichiro
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 82 Issue: 9 Pages: 1591-1598
  • DOI: 10.1080/09168451.2018.1478715
  • Peer Reviewed
• [Journal Article] The exercise-inducible bile acid receptor Tgr5 improves skeletal muscle function in mice (2018)
  • Author(s): T. Sasaki, A. Kuboyama, M. Mita, S. Murata, M. Shimizu, J. Inoue, K. Mori, and R. Sato
  • Journal Title: J. Biol. Chem. Volume: 293 Issue: 26 Pages: 10322-10332
  • DOI: 10.1074/jbc.ra118.002733
  • Peer Reviewed / Open Access
• [Journal Article] The expression of Transmembrane Protein 100 is regulated by alterations in calcium signaling rather than endoplasmic reticulum stress (2018)
  • Author(s): Ayane Kuboyama, Takashi Sasaki, Makoto Shimizu, Jun Inoue & Ryuichiro Sato
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 印刷中 Issue: 8 Pages: 1377-1383
  • DOI: 10.1080/09168451.2018.1464899
  • Peer Reviewed
• [Journal Article] The flavonoid derivative 4'-nitro-6-hydroxyflavone suppresses the activity of HNF4α and stimulates the degradation of HNF4α protein through the activation of AMPK. (2017)
  • Author(s): Inoue, J., Ikeda, S., Kanayama, T., and Sato, R.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 81 Issue: 8 Pages: 1548-1552
  • DOI: 10.1080/09168451.2017.1325316
  • Peer Reviewed
• [Journal Article] Identification of key amino acid residues in the hTGR5-nomilin interaction and construction of its binding model. (2017)
  • Author(s): Sasaki, T., Mita, M., Ikari, N., Kuboyama, A., Hashimoto, S., Kaneko, T., Ishiguro, M., Shimizu, M., Inoue, J., and Sato, R.
  • Journal Title: PLoS ONE Volume: 12 Issue: 6 Pages: e0179226-e0179226
  • DOI: 10.1371/journal.pone.0179226
  • Peer Reviewed / Open Access
• [Journal Article] Heat Shock Protein 90 Modulates Lipid Homeostasis by Regulating the Stability and Function of Sterol Regulatory Element-Binding Protein (SREBP) and SREBP Cleavage-Activating Protein. (2017)
  • Author(s): Kuan, Y-C., Hashidume, T., Shibata, T., Uchida, K., Shimizu, M., Inoue, J., and Sato, R.
  • Journal Title: J. Biol. Chem. Volume: 292 Issue: 7 Pages: 3106-3028
  • DOI: 10.1074/jbc.m116.767277
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] BCL11B gene heterozygosity causes weight loss accompanied by increased energy consumption, but not defective adipogenesis, in mice. (2017)
  • Author(s): Inoue, J., Ihara, Y., Tsukamoto, D., Yasumoto, K., Hashidume, T., Kamimura, K., Hirano, S., Shimizu, M., Kominami, R., and Sato, R.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 81 Issue: 5 Pages: 922-930
  • DOI: 10.1080/09168451.2016.1274642
  • Peer Reviewed
• [Journal Article] Identification of BCL11B as a regulator of adipogenesis. (2016)
  • Author(s): Inoue, J., Ihara, Y., Tsukamoto, D., Yasumoto, K., Hashidume, T., Kamimura, K., Nakai, Y., Hirano, S., Shimizu, M., Kominami, R., and Sato R.
  • Journal Title: Sci. Rep. Volume: 6 Issue: 1 Pages: 32750-32750
  • DOI: 10.1038/srep32750
  • Peer Reviewed / Open Access
• [Journal Article] Kaempferol stimulates gene expression of low-density lipoprotein receptor through activation of Sp1 in cultured hepatocytes. (2016)
  • Author(s): Ochiai, A., Miyata, S., Iwase, M., Shimizu, M., Inoue, J., and Sato, R.
  • Journal Title: Sci. Rep. Volume: 6 Issue: 1 Pages: 24940-24940
  • DOI: 10.1038/srep24940
  • Peer Reviewed / Open Access
• [Presentation] 再摂食時に発現が変動する遺伝子の網羅的解析 (2019)
  • Author(s): 渡邉 杏子、岩瀬 将盛、鈴木 司、山本 祐司、井上 順
  • Organizer: 日本農芸化学会2019年度大会／東京
• [Presentation] エピゲノム制御による摂食応答遺伝子の発現変動メカニズムの解析 (2019)
  • Author(s): 渡邉 杏子、岩瀬 将盛、鈴木 司、山本 祐司、井上 順
  • Organizer: 第73回日本栄養・食糧学会大会／静岡
• [Presentation] 抑制性転化因子BCL11Bによる脂肪細胞分化および脂肪蓄積制御の解析 (2017)
  • Author(s): 井上順
  • Organizer: 2017年度日本農芸化学会大会
  • Place of Presentation: 京都女子大（京都府京都市）
  • Year and Date: 2017-03-17
• [Presentation] 転写因子SREBP活性を抑制する食品成分の探索と分子機構の解析 (2017)
  • Author(s): 井上 順
  • Organizer: 第71回日本栄養・食糧学会大会