• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Constitutive and structural changes of membrane microdomains and lipid accumulation control by food chemicals

Research Project

Project/Area Number 16K14928
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Food science
Research InstitutionOkayama University

Principal Investigator

NAKAMURA Yoshimasa  岡山大学, 環境生命科学研究科, 教授 (60324381)

Co-Investigator(Kenkyū-buntansha) 加藤 陽二  兵庫県立大学, 環境人間学部, 教授 (30305693)
中村 俊之  岡山大学, 環境生命科学研究科, 助教 (90706988)
Research Collaborator LIU Xiaoyang  
YANG Qifu  
LIANG Ying  
SASAKI Ikumi  
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords食品 / 生体分子 / 分析化学 / 生化学 / 膜マイクロドメイン / インスリン / イソチオシアネート
Outline of Final Research Achievements

The purpose of this study was to clarify the physiological significance of the constitutive and structural changes of membrane microdomains by food chemicals through studies on their modulation of insulin signaling activation and its consequent phenomena such as the cancer cell survival and lipid accumulation. We identified benzyl isothiocyanate (BITC) as an insulin signal modulator. BITC enhanced glucose uptake in adipocytes as well as the survival pathway and autophagy in colorectal cancer cells, possibly through the activation of insulin signaling. The anti-cancer effect of BITC was potentiated by insulin signaling inhibitors as well as methyl-β-cyclodextrin, a depleting agent of membrane cholesterol.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (15 results)

All 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (4 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 4 results) Presentation (9 results) (of which Int'l Joint Research: 2 results,  Invited: 4 results) Book (1 results)

  • [Int'l Joint Research] Dalian Polytechnic University(China)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Physiological relevance of covalent protein modification by dietary isothiocyanates2018

    • Author(s)
      Nakamura Toshiyuki、Abe-Kanoh Naomi、Nakamura Yoshimasa
    • Journal Title

      Journal of Clinical Biochemistry and Nutrition

      Volume: 62 Issue: 1 Pages: 11-19

    • DOI

      10.3164/jcbn.17-91

    • NAID

      130006301253

    • ISSN
      0912-0009, 1880-5086
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of phosphatidylinositide 3-kinase ameliorates antiproliferation by benzyl isothiocyanate in human colon cancer cells2017

    • Author(s)
      Liu Xiaoyang、Takano Chiaki、Shimizu Tomomi、Yokobe Shintaro、Abe-Kanoh Naomi、Zhu Beiwei、Nakamura Toshiyuki、Munemasa Shintaro、Murata Yoshiyuki、Nakamura Yoshimasa
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 491 Issue: 1 Pages: 209-216

    • DOI

      10.1016/j.bbrc.2017.07.078

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Inhibition of phosphatidylinositide 3-kinase impairs the benzyl isothiocyanate-induced accumulation of autophagic molecules and Nrf2 in human colon cancer cells2017

    • Author(s)
      Liu Xiaoyang、Abe-Kanoh Naomi、Liu Yujia、Zhu Beiwei、Munemasa Shintaro、Nakamura Toshiyuki、Murata Yoshiyuki、Nakamura Yoshimasa
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 81 Issue: 11 Pages: 2212-2215

    • DOI

      10.1080/09168451.2017.1374830

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 3,4-Dihydroxyphenylacetic acid is a predominant biologically-active catabolite of quercetin glycosides.2016

    • Author(s)
      Tang, Y., Nakashima, S., Saiki, S., Myoi, Y., Abe, N., Kuwazuru, S., Zhu, B., Ashida, H., Murata, Y., Nakamura, Y.
    • Journal Title

      Food Res. Int.

      Volume: 89 Pages: 716-723

    • DOI

      10.1016/j.foodres.2016.09.034

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Benzyl isothiocyanate enhances glucose uptake and lipid accumulation via PI3K/Akt signaling pathway in 3T3-L1 adipocytes2018

    • Author(s)
      Ying Liang、佐々木郁美、竹田有希、中村俊之、宗正晋太郎、村田芳行、中村宜督
    • Organizer
      日本農芸化学会中四国支部第50回記念講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Methyl-β-cyclodextrin potentiated BITC-induced anti-cancer effect through modulation of the survival pathway in human colorectal cancer cells2018

    • Author(s)
      Qifu Yang、中村 俊之、宗正 晋太郎、村田 芳行、中村 宜督
    • Organizer
      日本農芸化学会中四国支部第50回記念講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ベンジルイソチオシアネートの脂肪蓄積抑制作用とその分子機構2018

    • Author(s)
      佐々木郁実、Liang Ying、竹田有希、中村俊之、宗正晋太郎、村田芳行、中村宜督
    • Organizer
      日本農芸化学会中四国支部第50回記念講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] イソチオシアネートの大腸がん細胞増殖抑制作用とその分子標的2017

    • Author(s)
      中村宜督
    • Organizer
      第13回レドックス・ライフイノベーションシンポジウム
    • Place of Presentation
      奈良
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Activation of phosphatidylinositol 3-kinase/Akt pathway plays a negative role in anti-proliferative effect of benzyl isothiocyanate.2017

    • Author(s)
      Xiaoyang Liu, Chiaki Takano, Shintaro Munemasa, Toshiyuki Nakamura, Yoshiyuki Murata, Yoshimasa Nakamura
    • Organizer
      日本農芸化学会中四国第47回講演会
    • Place of Presentation
      松江
    • Year and Date
      2017-01-28
    • Related Report
      2016 Research-status Report
  • [Presentation] フラボノイド配糖体代謝物の生体防御賦活作用2017

    • Author(s)
      中村宜督
    • Organizer
      日本農芸化学会中四国支部第48回講演会(例会)シンポジウム「生物資源を活用した応用研究」
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Phenolic acid catabolites are predominant biologically active compounds derived from quercetin glycosides2017

    • Author(s)
      Yoshimasa Nakamura
    • Organizer
      8th International Conference on polyphenols and Health (ICPH2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] A link between benzyl isothiocyanate-induced autophagy and Nrf2/Keap1 regulation in human colon cancer cells2017

    • Author(s)
      Xiaoyang Liu, Naomi Abe-Kanoh, Yujia Liu, Shintaro Munemasa, Toshiyuki Nakamura, Yoshiyuki Murata, Yoshimasa Nakamura
    • Organizer
      日本農芸化学会 2017年度関西・中四国・西日本支部合同大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Identification of Novel Targets for Antiproliferation by Benzyl Isothiocyanate in Colorectal Cancer Cells2016

    • Author(s)
      Yoshimasa Nakamura, Naomi Abe-Kanoh
    • Organizer
      The 29th Annual and International Meeting of Japanese Association for Animal Cell Technology (JAACT 2016)
    • Place of Presentation
      Kobe
    • Year and Date
      2016-11-09
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Book] 食べ物と健康 I.食品学総論 食品の成分と機能 2章食品の機能 1-3 脂質2017

    • Author(s)
      中村宜督
    • Total Pages
      11
    • Publisher
      中山書店
    • ISBN
      9784521742878
    • Related Report
      2017 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi