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Development of Poison Analysis Chip for Puffer Fish Liver and Exploration of New Food Science Field

Research Project

Project/Area Number 16K14930
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Food science
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Kawai Takayuki  国立研究開発法人理化学研究所, 生命機能科学研究センター, 研究員 (60738962)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsテトロドトキシン / フグ / キャピラリー電気泳動 / 質量分析 / 非接触型電気伝導率検出器 / オンサイト分析 / 肝臓 / 非接触型電気伝導度検出器 / ポータブルデバイス / 電気伝導度検出器 / 食品分析 / 現場解析 / マイクロチップ / 電気泳動 / 食品 / 分析科学
Outline of Final Research Achievements

Puffer fish liver is known to be delicious but contains quite toxic tetrodotoxin (TTX) and is inedible. Oppositely, farm-raised puffer fish has no TTX. If we could quantify TTX and certify no toxicity on site, such discarded food would be utilized as beneficial food.
In this study, capillary electrophoresis (CE) and capacitively coupled contactless conductivity detector (C4D) were coupled to develop portable TTX analysis device, which was successfully employed for TTX quantitation. We also employed CE coupled with sensitive mass spectrometry (MS) and measured localization of TTX in puffer fish liver. We found more than 10-times different concentration of TTX within the same liver tissue for the first time.

Academic Significance and Societal Importance of the Research Achievements

本研究成果により,美味だが可食とされてこなかったフグ肝臓をオンサイトで無毒と証明し,食用利用することができる道筋が開かれた。近年食用が禁止されたサバフグ類や養殖フグでは肝臓にTTXを含まないことが経験的に知られているため,非常に美味で貴重な食材が無駄となっていたが,本手法により有効利用が可能になり,新たな水産業価値を創出することに繋がると期待される。また本手法はフグ肝臓以外の食材等にも利用可能であり,将来的にあらゆる食べ物の有毒性をチェックして安全安心な社会を実現することにも繋がると考えられる。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (2 results)

All 2019

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (1 results) (of which Invited: 1 results)

  • [Journal Article] Ultrasensitive Single Cell Metabolomics by Capillary Electrophoresis-Mass Spectrometry with a Thin-Walled Tapered Emitter and Large-Volume Dual Sample Preconcentration2019

    • Author(s)
      Kawai Takayuki、Ota Nobutoshi、Okada Kaori、Imasato Akiko、Owa Yuri、Morita Makiko、Tada Misa、Tanaka Yo
    • Journal Title

      Analytical Chemistry

      Volume: 91 Issue: 16 Pages: 10564-10572

    • DOI

      10.1021/acs.analchem.9b01578

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] 超高感度キャピラリー電気泳動を用いた微量オミックス分析2019

    • Author(s)
      川井隆之
    • Organizer
      日本プロテオーム学会2019年大会・第70回日本電気泳動学会総会 合同大会
    • Related Report
      2019 Annual Research Report
    • Invited

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Published: 2016-04-21   Modified: 2021-02-19  

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