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Development of efficient production technique for pine wood nematode-resistant seedlings derived from embryogenic cells

Research Project

Project/Area Number 16K14949
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Forest science
Research InstitutionForest Research and Management Organization

Principal Investigator

Maruyama Tsuyoshi  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (20353865)

Co-Investigator(Kenkyū-buntansha) 細井 佳久  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (50353842)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords胚性万能細胞 / マツノザイセンチュウ抵抗性苗 / 不定胚形成 / クロマツ / 胚培養 / 体細胞分化 / 外気順化 / プロトプラスト培養 / マイクロプロパゲーション / 大量増殖 / マツノザイセンチュウ抵抗性 / 細胞培養
Outline of Final Research Achievements

Fourteen lines of embryogenic cells were induced from 3 pine wood nematode-resistant black pine families, and lines with high somatic embryo differentiation ability were selected. The induction rate of mature somatic embryos was significantly different depending on the cell line, but in the top five lines with high induction rates, more than 180 somatic embryos were formed per plate. When the resulting mature somatic embryos were desiccated for about 10 to 20 days and then cultured in a germination medium, they exhibited a germination rate of 68 to 93%. Thereafter, about 80 to 90% of the germinated somatic embryos formed plants. By carrying out stepwise acclimatization of the in vitro seedlings to the external air environment, 90% or more survival rates were obtained. In addition, protoplast culture of embryogenic cells revealed that somatic embryo induction is possible.

Academic Significance and Societal Importance of the Research Achievements

マツノザイセンチュウによる松枯れ被害対策の一環として、抵抗性個体の大量増殖が望まれている。これまで、実生や挿し木による苗生産が主に行われているが、これらの方法では増殖に時間と手間がかかり、大規模植林への実用的な対応が難しいという問題がある。そこで、組織培養により、不定胚形成能力を持つ胚性万能細胞から苗の大量生産技術を開発することで、マツノザイセンチュウ抵抗性苗を安定的かつ効率的に作出するための条件を明らかにし、大きな問題となっている松枯れ被害の対策として、実用化に向けた基盤技術の開発に貢献できる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (19 results)

All 2019 2018 2017 2016

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (12 results) (of which Int'l Joint Research: 4 results,  Invited: 3 results) Book (2 results) Funded Workshop (1 results)

  • [Journal Article] Progress in somatic embryogenesis of Japanese pines2019

    • Author(s)
      MARUYAMA Tsuyoshi, HOSOI Yoshihisa
    • Journal Title

      Frontiers in Plant Science

      Volume: 10:31 Pages: 1-15

    • DOI

      10.3389/fpls.2019.00031

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 海岸林再生に向けた不定胚形成によるマツノザイセンチュウ抵抗性クロマツ家系の苗木増殖2017

    • Author(s)
      大西昇、安野紀子、丸山毅、今野幸則、山野邉太郎、織部雄一朗
    • Journal Title

      森林遺伝育種

      Volume: 第6巻 Pages: 64-68

    • NAID

      130007834491

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Somatic embryogenesis and plant regeneration in Japanese pines and cypresses2017

    • Author(s)
      MARUYAMA Tsuyoshi, HOSOI Yoshihisa
    • Journal Title

      Proceedings of the 4th International Conference of the IUFRO Unit 2.09.02 September 19-23, 2016. La Plata, Argentina

      Volume: 4th Pages: 340-340

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] ヤクタネゴヨウ、クロマツの針葉・芽切片からのカルス誘導と細胞培養2017

    • Author(s)
      細井佳久、丸山毅
    • Journal Title

      関東森林研究

      Volume: 第68(1)巻 Pages: 1-4

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] Somatic embryogenesis and plant regeneration in Japanese pines2019

    • Author(s)
      MARUYAMA Tsuyoshi, HOSOI Yoshihisa
    • Organizer
      Plant 2019, Conference on Plant, Cellular & Molecular Biology, Valencia, Spain
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Progress in somatic embryogenesis of Japanese conifers2018

    • Author(s)
      MARUYAMA Tsuyoshi, HOSOI Yoshihisa
    • Organizer
      IUFRO Working Party 2.09.02, Somatic Embryogenesis and Other Vegetative Propagation Technologies, Coimbra, Portugal
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Propagation of Ryukyu pine (Pinus luchuensis Mayr.) through somatic embryogenesis2018

    • Author(s)
      MARUYAMA Tsuyoshi, HOSOI Yoshihisa
    • Organizer
      In Vitro Cellular & Developmental Biology, Congress of the IAPB, Dublin, Ireland
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マツノザイセンチュウ抵抗性クロマツ不定胚形成細胞からの成熟不定胚誘導2018

    • Author(s)
      丸山毅、細井佳久、倉本哲嗣、今野幸則
    • Organizer
      第129回日本森林学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] マツノザイセンチュウ抵抗性クロマツ種子からの不定胚形成細胞の誘導2017

    • Author(s)
      丸山毅、細井佳久、今野幸則、倉本哲嗣
    • Organizer
      第128回日本森林学会大会
    • Place of Presentation
      鹿児島大学農学部(鹿児島県、鹿児島市)
    • Year and Date
      2017-03-26
    • Related Report
      2016 Research-status Report
  • [Presentation] リュウキュウマツ、ヤクタネゴヨウ組織から誘導したカルスからのプロトプラストの単利・培養2017

    • Author(s)
      細井佳久、丸山毅
    • Organizer
      第128回日本森林学会大会
    • Place of Presentation
      鹿児島大学農学部(鹿児島県、鹿児島市)
    • Year and Date
      2017-03-26
    • Related Report
      2016 Research-status Report
  • [Presentation] 植物組織培養技術によるクロマツ林の再生への取組2017

    • Author(s)
      大西昇、安野紀子、丸山毅、今野幸則、山野邉太郎、織部雄一朗
    • Organizer
      第35回日本植物細胞分子生物学会大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 材線虫抵抗性クロマツの種子胚由来細胞の培養と分化2017

    • Author(s)
      細井佳久、丸山毅
    • Organizer
      第81回日本植物学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Somatic embryogenesis and plant regeneration in Japanese pines and cypresses2016

    • Author(s)
      MARUYAMA Tsuyoshi, HOSOI Yoshihisa
    • Organizer
      4th International Conference of the IUFRO Working Party 2.09.02 Somatic Embryogenesis and Other Vegetative Propagation Technologies
    • Place of Presentation
      Universidad Nacional de La Plata (Buenos Aires, Argentina)
    • Year and Date
      2016-09-19
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] マツ材線虫病により減少するヤクタネゴヨウの無菌培養による増殖の試み2016

    • Author(s)
      細井佳久、丸山毅
    • Organizer
      第80回日本植物学会大会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県、宜野湾市)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Research-status Report
  • [Presentation] マツノザイセンチュウ抵抗性クロマツ採種園産種子からの不定胚形成細胞誘導の系統間差2016

    • Author(s)
      大西昇、安野紀子、飯田綾加、丸山毅、今野幸則、山野邉太郎、織部雄一朗
    • Organizer
      第34回日本植物細胞分子生物学会大会
    • Place of Presentation
      信州大学繊維学部(長野県、上田市)
    • Year and Date
      2016-09-01
    • Related Report
      2016 Research-status Report
  • [Presentation] ヤクタネゴヨウ、クロマツの針葉・芽切片からのカルス誘導と細胞培養2016

    • Author(s)
      細井佳久、丸山毅
    • Organizer
      第6回関東森林学会大会
    • Place of Presentation
      千葉市民会館(千葉県、千葉市)
    • Related Report
      2016 Research-status Report
  • [Book] Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants. Protocol for somatic embryogenesis in Japanese black pine (Pinus thunbergii Parl.) and Japanese red pine (Pinus densiflora Sieb. et Zucc.)2018

    • Author(s)
      JAIN M, GUPTA P (Eds), MARUYAMA Tsuyoshi, HOSOI Yoshihisa
    • Total Pages
      323
    • Publisher
      Springer
    • ISBN
      9783319894829
    • Related Report
      2018 Annual Research Report
  • [Book] Micropropagation of Ornamental Plants. Propagation of Ryukyumatsu (Pinus luchuensis Mayr.) via somatic embryogenesis2018

    • Author(s)
      BETTAIEB T (Ed), HOSOI Yoshihisa, MARUYAMA Tsuyoshi
    • Total Pages
      214
    • Publisher
      Nova Science Publishers
    • ISBN
      9781536145410
    • Related Report
      2018 Annual Research Report
  • [Funded Workshop] 4th International Conference of the IUFRO Working Party 2.09.02 Somatic Embryogenesis and Other Vegetative Propagation Technologies2016

    • Place of Presentation
      Universidad Nacional de La Plata (Buenos Aires, Argentina)
    • Year and Date
      2016-09-19
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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