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Identification of key peptides which enable to achieve the molecular breeding of brown-rot fungi

Research Project

Project/Area Number 16K14952
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Wood science
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

YOSHIDA Makoto  東京農工大学, (連合)農学研究科(研究院), 准教授 (30447510)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords木材腐朽 / 植物細胞壁分解 / 褐色腐朽
Outline of Final Research Achievements

Brown-rot fungi has been considered as microorganisms which are able to remove plant cell wall polysaccharides, such as cellulose and hemicelluloses, together with extensive modification of lignin structure, but without lignin metabolism. The wood degradation mechanism would be used for the development of new saccharification process which does not require the de-lignification process.
We focused that peptides and low-molecular proteins with iron-binding ability, which has been considered to be involved in wood degradation system by brown rot fungi. In the present study, we selected and analyzed the peptides and low-molecular proteins produced by brown rot fungus in order to identify the key factor which enable us to control the brown rot system.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (15 results)

All 2018 2017 2016 Other

All Int'l Joint Research (2 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (11 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results)

  • [Int'l Joint Research] Norwegian University of Life Sciences(Norway)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] University of Massachusetts(米国)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] 腐朽メカニズムの概要と研究の展望2018

    • Author(s)
      吉田 誠
    • Journal Title

      木材保存

      Volume: In press

    • NAID

      130007428921

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Characterization of an LPMO from the brown-rot fungus Gloeophyllum trabeum with broad xyloglucan specificity, and its action on cellulose-xyloglucan complexes2016

    • Author(s)
      Kojima, Y., Varnai, A., Ishida, T., Sunagawa, N., Petrovic, D., Igarashi, K., Jellison, J., Goodell, B., Alfredsen, G., Westereng, B., Eijsink, V., and Yoshida M.
    • Journal Title

      Appl. Environ. Microbiol.

      Volume: 82 Issue: 22 Pages: 6557-6572

    • DOI

      10.1128/aem.01768-16

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 菌類による植物バイオマス分解機構について新たにわかってきたこと2018

    • Author(s)
      吉田 誠
    • Organizer
      セルロース学会北海道・東北支部セミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 褐色腐朽菌Gloeophyllum trabeumから見出された新規セルロース結合モジュールの機能解析2018

    • Author(s)
      小嶋由香、吉田誠、砂川直輝、五十嵐圭日子
    • Organizer
      第68回日本木材学会年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 進化系統的に異なる分岐群に属する褐色腐朽菌間での木材分解様式の比較2018

    • Author(s)
      近藤里沙子、安藤恵介、半 智史、堀川祥生、吉田 誠
    • Organizer
      第68回日本木材学会年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 褐色腐朽菌 Gloeophyllum trabeum が有する糖質関連酵素の網羅的遺伝子発現解析2017

    • Author(s)
      新倉 舞, 梅澤 究, 小瀬亮太, 堀川祥生, 吉田 誠
    • Organizer
      第67回日本木材学会年次大会
    • Place of Presentation
      アクロス福岡(福岡)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] 褐色腐朽菌 Gloeophyllum trabeum の遺伝子組換え技術を利用したレポーター遺伝子の発現2017

    • Author(s)
      渡部翔一、梅澤 究、吉田 誠
    • Organizer
      第67回日本木材学会年次大会
    • Place of Presentation
      アクロス福岡(福岡)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] Auxiliary Activities Family 12: unique PQQ-dependent redox enzymes2017

    • Author(s)
      Makoto Yoshida
    • Organizer
      Gordon Research Conference -Cellulases & Other Carbohydrate-Active Enzymes-
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Characterization of an LPMO9 acting on cellulose and hemicellulose from the brown-rot fungus Gloeophyllum trabeum2017

    • Author(s)
      Yuka Kojima、Aniko Varnai、Takuya Ishida、Naoki Sunagawa、Dejan M. Petrovic、Kiyohiko Igarashi、Jody Jellison、Barry Goodell、Gry Alfredsen、Bjorge Westereng、Vincent G. H. Eijsink、Makoto Yoshida
    • Organizer
      Gordon Research Conference -Cellulases & Other Carbohydrate-Active Enzymes-
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 褐色腐朽菌 Gloeophyllum trabeum 由来 LPMO9 のセルロースおよびヘミセルロース分解特性2017

    • Author(s)
      小嶋由香、Aniko Varnai、Dejan Petrovic、Borge Westereng、Vincent Eijsink、石田卓也、砂川直輝、五十嵐圭日子、Jody Jellison、Barry Goodell、Gry Alfredsen、吉田誠
    • Organizer
      日本応用糖質科学会平成29年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 腐朽菌の木材分解メカニズムを分子レベルで理解する2017

    • Author(s)
      吉田 誠
    • Organizer
      日本木材学会生物劣化研究会 2017 年春季研究会
    • Place of Presentation
      九州大学箱崎キャンパス(福岡)
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Characterization of an LPMO9 from the brown-rot fungus Gloeophyllum trabeum.2016

    • Author(s)
      Yuka Kojima, Aniko Varnai, Takuya Ishida, Naoki Sunagawa, Dejan M. Petrovic, Kiyohiko Igarashi, Jody Jellison, Barry Goodell, Gry Alfredsen, Bjorge Westereng, Vincent G.H. Eijsink, and Makoto Yoshida
    • Organizer
      LPMO Symposium
    • Place of Presentation
      Copenhagen (デンマーク)
    • Year and Date
      2016-11-15
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 褐色腐朽菌Gloeophyllum trabeum由来LPMO9の多糖分解特性2016

    • Author(s)
      小嶋由香,吉田誠,Aniko Varnai,Dejan Petrovic,Borge Westereng,Vincent Eijsink,石田卓也,砂川直輝,五十嵐圭日子,Jody Jellison,Barry Goodell,Gry Alfredsen
    • Organizer
      第30回セルラーゼ研究会
    • Place of Presentation
      佐久平プラザ21(長野)
    • Year and Date
      2016-07-08
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2022-06-07  

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