Exploration of fish eustress / distress using wireless biosensor system

• Project/Area Number: 16K14969
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Aquatic bioproduction science
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: Hideaki Endo 東京海洋大学, 学術研究院, 教授 (50242326)
• Research Collaborator: Arimoto Takafumi 東京海洋大学, 学術研究院, 名誉教授 (20106751) ; Nakano Toshiki 東北大学大学院, 農学研究科, 助教 (10217797)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: バイオセンサ / 魚類 / ストレス / ユーストレス / ディストレス / グルコース / バイオセンシング / モニタリング

Outline of Final Research Achievements

We aimed to clarify the relationship between beneficial stress (eustress) and harmful stress (distress) in fish using a biosensor system to evaluate the stress response. We first developed a biosensor system suitable for our purposes. Next, the test fish (Nile tilapia) were bred with lights of different wavelengths (white, blue, red, or green), and recovery from stress was monitored over time. Breeding under green wavelengths tended to be most effective for recovery. Also, we examined the effect of the color of the inner wall of the fish tank (blue, red, or green) on recovery from stress. The pattern of recovery was also monitored which similar to that under different wavelengths. The red inner wall showed relatively effective for recovery from stress in the test fish compared to the rest color. These findings may be associated with eustress, but differences in the response among individuals were also observed.

Academic Significance and Societal Importance of the Research Achievements

本研究では，これまで未知のテーマであった魚類におけるユーストレス／ディストレスの存在について，バイオセンサを用いることによりその解明を試みた．この手法の特色は，従来のバイオロギングや採血を伴う測定法では解明することが困難であった「魚を泳がせながらストレス応答をリアルタイムにモニタリングできる」という点である．本研究の遂行により，魚類のユーストレスの存在に関する学術的解明においての手がかりが得られたものと考えている．これらの成果は，魚類行動学，魚類生理学，水産化学，比較生理生化学等の分野に対して，横断的発展のための新知見を提供できる可能性があり，その意義は極めて大きいと考えている．

Research Products

• [Journal Article] Development of a Novel Enhanced Biosensor System for Real-Time Monitoring of Fish Stress Using a Self-Assembled Monolayer (2019)
  • Author(s): H. Wu, Y. Fujii, T. Nakano,T. Arimoto, M. Murata, H. Matsumoto,Y. Yoshiura, H. Ohnuki, and H. Endo
  • Journal Title: Sensors Volume: 19 Issue: 7 Pages: 1518-1518
  • DOI: 10.3390/s19071518
  • Peer Reviewed / Open Access
• [Journal Article] Some artificially applied stressors have a positive effect on fish fitness (2017)
  • Author(s): T. Nakano, H. Wu, T. Arimoto, H. Endo, T. Yamaguchi, Y. Ochiai
  • Journal Title: The JSFS 85th Anniversary-Commemorative International Symposium “Fisheries Science for Future Generations,” Symposium Proceedings Volume: - Pages: 06010-06010
• [Presentation] バイオセンサを用いた魚類のユーストレスの探求～水槽内面の色が及ぼす影響～ (2019)
  • Author(s): 山本航大・呉 海雲・有元貴文・中野俊樹・村田政隆・松本陽斗・遠藤英明
  • Organizer: 平成31年度日本水産学会春季大会
• [Presentation] 自己組織化単分子膜を用いた魚類のためのグルコース測定用バイオセンサの試作に関する研究 (2017)
  • Author(s): 藤井柚・山田和輝・呉海雲・大貫等・日比香子・遠藤英明
  • Organizer: 平成29年度日本水産学会秋春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2017-03-26
• [Presentation] Exploration of fish eustress using wireless biosensor system (2017)
  • Author(s): H. Takahashi, M. Nakayama, H. Wu, T. Arimoto, T. Nakano, H. Endo
  • Organizer: International Symposium “Fisheries Science for Future Generations”
  • Int'l Joint Research
• [Presentation] Some artificially applied stressors have a positive effect on fish fitness (2017)
  • Author(s): T. Nakano, H. Wu, T. Arimoto, H. Endo, T. Yamaguchi, Y. Ochiai
  • Organizer: International Symposium “Fisheries Science for Future Generations”
  • Int'l Joint Research