| Project/Area Number |
16K14972
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Aquatic bioproduction science
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| Research Institution | Tokyo University of Marine Science and Technology |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 細菌性疾病 / 耐病性 / ゲノム / 育種 / アユ / SNP / 次世代シーケンサー / 相関解析 / 冷水病 / 感染実験 |
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| Outline of Final Research Achievements |
The reference genome (approximately, 460 Mbp) of the ayu was produced. Longest scaffold was 17.19 Mbp and the assembly had an N50 value of 4.44 Mbp. In order to detect the genetic impacts for the selective breeding program (SBP) of bacterial coldwater-disease (BCWD) resistant trait, allele distributions of MS markers were compared in two years (2007 and 2016). The allele distributions of several markers were significantly difference between 2007 and 2016, suggesting the genetic diversity was reduced due to SBP. The difference of the allele distribution on MS marker linked to BCDW resistant trait was highly significant, because of the genetic selection on the SBP. Genome wide association study (GWAS) was performed in wild fish after artificial challenge test of BCWD. The genetic difference between survivors and dead fish on the genome region related to BCWD resistant trait was detected. GWAS of disease resistant trait might be useful to detect the disease resistant locus.
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