• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Search for novel ovarian factors that dominantly regulate ovulation number

Research Project

Project/Area Number 16K15023
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Animal production science
Research InstitutionThe University of Tokyo

Principal Investigator

Matsuda Fuko  東京大学, 大学院農学生命科学研究科(農学部), 准教授 (10608855)

Research Collaborator OHKURA satoshi  
MAEDA kei-ichiro  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords獣医学 / 畜産学 / 応用動物 / 細胞・組織 / プロテオーム
Outline of Final Research Achievements

The ovulation number is strictly controlled depending on species in mammals, but the mechanism is still unclear. In this study, I performed transcriptome analysis (RNA-seq) using bovine ovarian granulosa cells to search for the factors that dominantly regulate ovulation number. As a result, I identified more than 500 genes which expression levels are significantly different between granulosa cells of dominant follicles (the biggest follicle that will be ovulated) and subordinate follicles (the second biggest follicle that will not be ovulated). I choose 3 factors as candidate factors that regulate ovulation number and performed detailed morphological analysis confirming similar results with the RNA-seq analysis.

Academic Significance and Societal Importance of the Research Achievements

本研究では排卵数の決定に関わる可能性がある複数の因子を同定することに成功した。これらの因子の機能を促進あるいは抑制することで、家畜やヒトの排卵数を任意に調節できるようになる可能性がある。それによって、双子の出産が問題となるウシでは双胎の回避、ブタでは産子数を増やすことによる生産効率向上、卵巣機能障害を持つ家畜やヒトにおいてはその治療などへの応用が可能となり、獣医畜産・医療分野へ大きく貢献することが期待される。

Report

(3 results)
  • 2018 Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (9 results)

All 2017 2016

All Journal Article (2 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (7 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] 中枢からのアプローチ:新たな神経内分泌メカニズムを利用した繁殖制御2017

    • Author(s)
      松田二子、前多敬一郎、大蔵 聡、上野山賀久、束村博子
    • Journal Title

      臨床獣医

      Volume: 35 Pages: 98-101

    • Related Report
      2017 Research-status Report
  • [Journal Article] Immunohistochemical characterization of the arcuate kisspeptin/neurokinin B/dynorphin (KNDy) and preoptic kisspeptin neuronal populations in the hypothalamus during the estrous cycle in heifers.2016

    • Author(s)
      Hassaneen, A.S.A., Naniwa, Y., Suetomi, Y., Matsuyama, S., Kimura, K., Ieda, N., Inoue, N., Uenoyama, Y., Tsukamura, H., Maeda, K.-I., Matsuda, F. and Ohkura, S.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 62 Issue: 5 Pages: 471-477

    • DOI

      10.1262/jrd.2016-075

    • NAID

      130005431286

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 遺伝子改変ラットを用いたkappa opioid receptor (KOR) 発現細胞の解析2016

    • Author(s)
      高橋 宙大,後藤 哲平,平林 真澄,中村 翔,戴 明道,池上 花奈, 松田二子、上野山賀久,束村博子,前多 敬一郎
    • Organizer
      第43回日本神経内分泌学会学術集会
    • Place of Presentation
      アクトシティ浜松(静岡県浜松市)
    • Year and Date
      2016-10-14
    • Related Report
      2016 Research-status Report
  • [Presentation] シバヤギKNDyニューロン不死化細胞株の樹立2016

    • Author(s)
      末富祐太,舘林亮輝,束村博子,大蔵聡,松田二子
    • Organizer
      第109回日本繁殖生物学会大会
    • Place of Presentation
      麻布大学(東京都相模原市)
    • Year and Date
      2016-09-12
    • Related Report
      2016 Research-status Report
  • [Presentation] ラットキスペプチンニューロン不死化細胞株の樹立2016

    • Author(s)
      堀畑慶,家田菜穂子,井上直子,上野山賀久,末富祐太,松田二子,前多敬一郎,束村博子
    • Organizer
      第109回日本繁殖生物学会大会
    • Place of Presentation
      麻布大学(東京都相模原市)
    • Year and Date
      2016-09-12
    • Related Report
      2016 Research-status Report
  • [Presentation] Modification of KISS1 gene in goat embryonic fibroblasts using TALEN2016

    • Author(s)
      Ryoki Tatebayashi, Tetsushi Sakuma, Takashi Yamamoto, Satoshi Ohkura, Fuko Matsuda
    • Organizer
      International Symposium on Pituitary Gland and Related Systems 2016
    • Place of Presentation
      ホノルル(米国)
    • Year and Date
      2016-09-01
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Establishment of immortalized cell lines derived from cattle GnRH neurons2016

    • Author(s)
      Riho OZAKI, Yuta SUETOMI, Shuichi MATSUYAMA, Koji KIMURA, Satoshi OHKURA, Fuko Matsuda
    • Organizer
      International Symposium on Pituitary Gland and Related Systems 2016
    • Place of Presentation
      ホノルル(米国)
    • Year and Date
      2016-09-01
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Establishment of a Neuronal Cell Line Derived from KNDy Neuron in a Goat2016

    • Author(s)
      Yuta Suetomi, Ryoki Tatebayashi, Hiroko Tsukamura, Satoshi Ohkura, Fuko Matsuda
    • Organizer
      The 49th Annual Meeting of Society for the Study of Reproduction
    • Place of Presentation
      サンディエゴ(米国)
    • Year and Date
      2016-07-16
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 排卵中枢キスペプチンニューロンの性分化には種差がある2016

    • Author(s)
      松田二子、渡辺雄貴、大蔵聡、井上直子、上野山賀久、前多敬一郎、束村博子
    • Organizer
      第13回GPCR研究会
    • Place of Presentation
      日本科学未来館(東京都江東区)
    • Year and Date
      2016-05-13
    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi