Comprehensive analysis by 'Immunobiogenomics' for expanding the immunobiotic availability

• Project/Area Number: 16K15028
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Animal production science
• Research Institution: Tohoku University
• Principal Investigator: Kitazawa Haruki 東北大学, 農学研究科, 准教授 (10204885)
• Co-Investigator(Kenkyū-buntansha): 麻生 久 東北大学, 農学研究科, 教授 (50241625)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 畜産学 / イムノバイオティクス / 免疫学 / イムノバイオゲノミクス / トランスクリプトミクス / 食品 / 飼料 / 微生物 / 食品・飼料 / 動物

Outline of Final Research Achievements

In this study, we were able to build a certain base for elucidating a strain-specific mucosal immunoregulatory mechanisms of Immunobiotics by constructing an "Immunobiogenomics" in the interdisciplinary study field between bioinformatics and immunobiotics. Expansion for effective availability of Immunobiotics is expected and can thereby contribute to further studies and industrial development.

Research Products

• [Int'l Joint Research] CERELA-CONICET(Argentina)
• [Int'l Joint Research] CERELA-CONICET(Argentina)
• [Journal Article] Immunobiotics for the bovine host: their interaction with intestinal epithelial cells and their effect on antiviral immunity. (2018)
  • Author(s): Julio Villena, Hisashi Aso, Victor P. Rutten, Hideki Takahashi, Willem Van Eden and Haruki Kitazawa
  • Journal Title: Frontiers in Immunology Volume: 9 Pages: 326-326
  • DOI: 10.3389/fimmu.2018.00326
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Exopolysaccharides from Lactobacillus delbrueckii OLL1073R-1 modulate innate antiviral immune response in porcine intestinal epithelial cells (2017)
  • Author(s): Paulraj Kanmani, Leonardo Albarra2018.00326in, Hisakazu Kobayashi, Hikaru Iida, Ryoya Komatsu, A.K.M. Humayun Kober, Wakako Ikeda-Ohtsubo, Yoshihito Suda, Hisashi Aso, Seiya Makino, Hiroshi Kano, Tadao Saito, JulioVillen, Haruki Kitazawa
  • Journal Title: Molecular Immunology Volume: 93 Pages: 253-265
  • DOI: 10.1016/j.molimm.2017.07.009
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Peptidoglycan from immunobiotic Lactobacillus rhamnosus improves resistance of infant mice to respiratory syncytial viral infection and secondary pneumococcal pneumonia. (2017)
  • Author(s): Clua, P., P. Kanmani, H. Zelaya, A. Tada, AKM H. Kober, S. Salva, S. Alvarez, H. Kitazawa, J. Villena
  • Journal Title: Front. Immunol. Volume: 8(948) Pages: 1-15
  • DOI: 10.3389/fimmu.2017.00948
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Draft genome sequence of immunobiotic Lactobacillus rhamnosus IBL027 a potential adjuvant for mucosal vaccine development. (2017)
  • Author(s): Maria Guadalupe Vizoso-Pinto, M. G., L. Saavedra, E. Hebert, F. R. Tonetti, L. Albarracin, S. Alvarez, H. Kitazawa, J. Villena
  • Journal Title: Genome Announc. Volume: 5(50):e01268-17 Issue: 50 Pages: 1-2
  • DOI: 10.1128/genomea.01268-17
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Transcriptomic analysis of the innate antiviral immune response in porcine intestinal epithelial cells: influence of immunobiotic lactobacilli. (2017)
  • Author(s): Albarracin, L., H. Kobayashi, H. Iida, N. Sato, T. Nochi, H. Aso, S. Salva, S. Alvarez, H. Kitazawa, J. Villena
  • Journal Title: Front. Immunol. Volume: 8(57) Pages: 1-15
  • DOI: 10.3389/fimmu.2017.00057
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Draft genome sequence of Lactobacillus plantarum MPL16 a wakame-utilizing immunobiotic strain from swine feces. (2017)
  • Author(s): Villena, J., L. Saavedra, E. Hebert, Y. Suda, Y. Masumizu, L. Albarracin, P. Clua, W. Ikeda-Ohtsubo, H. Kitazawa
  • Journal Title: Genome Announc. Volume: 5(10): e00006-17 Issue: 10 Pages: 1-2
  • DOI: 10.1128/genomea.00006-17
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Draft genome sequence of the immunobiotic strain Lactobacillus jensenii TL2937. (2017)
  • Author(s): Villena, J.,Y. Masumizu, H. Ida, W. Ikeda-Ohtsubo, L. Albarracin, S. Makino, S. Ohkawara, K.i Kimura, L. Saavedra, E. Hebert, H. Kitazawa
  • Journal Title: Genome Announc. Volume: 5(9): e00005-17 Issue: 9 Pages: 1-2
  • DOI: 10.1128/genomea.00005-17
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937: finding new biomarkers for the selection of antiinflammatory immunobiotics. (2016)
  • Author(s): Kobayashi, H., L. Albarracin, N. Sato, P. Kanmani, AKM H. Kober, W. Ikeda-Ohtsubo, Y. Suda, T. Nochi, H. Aso, S. Makino, H. Kano, S. Ohkawara, T. Saito, J. Villena, H. Kitazawa
  • Journal Title: Beneficial Microbes, Benef Microbes Volume: 7 Issue: 5 Pages: 769-782
  • DOI: 10.3920/bm2016.0095
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Draft Genome Sequence of Lactobacillus plantarum TL2766, a strain with the ability to ferment wakame. (2016)
  • Author(s): Villena, J., L. Saavedra, E. Hebert, Y. Masumizu, N. Sato, AKM H. Kober, L. Albarracin, W. Ikeda-Ohtsubo, S. Makino, K. Kimura, S. Ohkawara, H. Kitazawa
  • Journal Title: Genome Announc. Volume: 4(6): e01328-16 Issue: 6 Pages: 1-2
  • DOI: 10.1128/genomea.01328-16
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Immunobiotic Lactobacillus strains reduce small intestinal injury induced by intraepithelial lymphocytes after Toll-like receptor 3 activation. (2016)
  • Author(s): Tada, A., H. Zelaya, P. Clua, S. Salva, S. Alvarez, H. Kitazawa, J. Villena
  • Journal Title: Inflamm. Res. Volume: 65(10) Issue: 10 Pages: 771-783
  • DOI: 10.1007/s00011-016-0957-7
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] ブタ腸管由来ワカメ資化性乳酸菌のイムノバイオティック利用性 (2017)
  • Author(s): 舛水由季、飯田ひかる、須田義人、大坪和香子、麻生 久、齋藤忠夫、鈴木啓一、北澤春樹
  • Organizer: 日本畜産学会第122回大会
  • Place of Presentation: 神戸大学（兵庫県神戸市）
  • Year and Date: 2017-03-28
• [Presentation] Lactobacillus delbrueckii OLL1073R-1由来菌体外多糖のブタ腸管上皮細胞における抗ウイルス自然免疫修飾 (2017)
  • Author(s): 小松稜弥、須田義人、牧野聖也、狩野 宏、齋藤忠夫、北澤春樹
  • Organizer: 日本食品免疫学会 第１３回学術大会
• [Presentation] "Immunosynbiotics" approach by using wakame and immunobiotics as livestock healthy growth strategy (2017)
  • Author(s): Nakano, Y., Y. Masumizu, AKM. H. Kober, V. Garcia, W. Ikeda-Ohtsubo, Y. Suda, K. Suzuki, T. Saito, J. Villena, H. Kitazawa
  • Organizer: 日本畜産学会第123回大会 (英語口頭発表)
• [Presentation] 畜産食品の安全性向上に寄与するブタ由来ワカメ資化性乳酸菌のイムノバイオティック利用性 (2016)
  • Author(s): 舛水由季、飯田ひかる、須田義人、齋藤忠夫、北澤春樹
  • Organizer: 日本食品免疫学会第12回学術大会
  • Place of Presentation: 東京大学（東京都文京区）
  • Year and Date: 2016-11-09
• [Presentation] Isolation and characterization of Lactobacillus plantarum MPL16: A Wakame-utilizing immunobiotic strain from swine feces. (2016)
  • Author(s): Masumizu, Y., AKM H. Kober, B. Tzu-An Chao, H. Iida, W. Ikeda-Ohtsubo, Y. Suda, L. Albarracin, L. Saavedra, E.M. Hebert, T. Nochi, H. Aso, T. Saito, K. Suzuki, J. Villena, H. Kitazawa
  • Organizer: V International Symposium on Lactic Acid Bacteria - Benefitting from lactic acid bacteria, Progress in health and food
  • Place of Presentation: Tucuman, Argentina
  • Year and Date: 2016-10-19
  • Int'l Joint Research
• [Presentation] ワカメ資化性イムノバイオティクスの応用基礎研究 (2016)
  • Author(s): 舛水由季、AKM H. Kober、B. Tzu-An Chao、飯田ひかる、大坪和香子、須田義人、麻生 久、齋藤忠夫、鈴木啓一、J. Villena、北澤春樹
  • Organizer: 2016年度酪農科学シンポジウム
  • Place of Presentation: 日本大学生物資源科学部（神奈川藤沢市）
• [Remarks] 食と農免疫国際教育研究センター
  • URL: http://www.agri.tohoku.ac.jp/cfai/
• [Remarks] JSPS研究拠点形成事業先端拠点形成「食の安全性の飛躍的向上を目指した農免疫国際共同研究拠点形成」
  • URL: http://www.agri.tohoku.ac.jp/fain/index.html