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Identification of MSC subpopulations that have therapeutic potential for neurodegenerative disorders

Research Project

Project/Area Number 16K15033
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Veterinary medical science
Research InstitutionHokkaido University

Principal Investigator

HORIUCHI MOTOHIRO  北海道大学, 獣医学研究科, 教授 (30219216)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords間葉系幹細胞 / プリオン病 / 神経変性疾患 / 自然免疫 / 治療効果 / 獣医学 / 感染症 / 脳神経疾患 / 再生医療 / プリオン
Outline of Final Research Achievements

Intra-cerebral transplantation of compact bone-derived MSCs (CB-MSCs) prolonged survival of prion-infected mice, suggesting that CB-MSCs could be useful for the treatment of neurodegenerative disorders. In the CB-MSCs transplanted prion-infected mice, unexpectedly, microglia were more activated than un-transplanted mice. However, up-regulation of some of the marker genes for M2-type microglia, such as Arg-1, suggesting that CB-MSCs modify the activation state of microglia. The culture supernatant of CB-MSCs also shifted the activation state of microglia recovered from prion-infected mice to M2-type activation state. These results suggest that the alteration of innate immunity by CB-MSCs is one of the mechanisms for the therapeutic effects of CB-MSCs on neurodegenerative disorders.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (18 results)

All 2018 2017 2016 Other

All Int'l Joint Research (2 results) Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 1 results,  Acknowledgement Compliant: 4 results) Presentation (10 results) (of which Int'l Joint Research: 9 results,  Invited: 2 results)

  • [Int'l Joint Research] Daegue Haany University(韓国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] ロッキー山研究所/国立衛生研究所(米国)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Flow cytometric detection of PrPSc in neurons and glial cells from prion-infected mouse brains2018

    • Author(s)
      Yamasaki T, Suzuki A, Hasebe R, Horiuchi M.
    • Journal Title

      Journal of Virology

      Volume: 92 Issue: 1

    • DOI

      10.1128/jvi.01457-17

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Therapeutic effect of autologous compact bone-derived mesenchymal stem cell transplantation on prion disease2017

    • Author(s)
      Shan Z, Hirai Y, Nakayama M, Hayashi R, Yamasaki T, Hasebe R, Song CH, Horiuchi, M
    • Journal Title

      J Gen Virol

      Volume: 98 Pages: 2615-2627

    • NAID

      120006523738

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] PrPSc-Specific Antibody Reveals C-Terminal Conformational Differences between Prion Strains2016

    • Author(s)
      Saijo E, Hughson AG, Raymond GJ, Suzuki A, Horiuchi M, and Caughey B
    • Journal Title

      J. Virol.

      Volume: 90 Pages: 4905-4913

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Complement factors alter the amount of PrP(Sc) in primary-cultured mouse cortical neurons associated with increased membrane permeability2016

    • Author(s)
      Hasebe R, Tanaka M, Suzuki A, Yamasaki T, Horiuchi M
    • Journal Title

      Virology

      Volume: 496 Pages: 9-20

    • DOI

      10.1016/j.virol.2016.05.014

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Comparison of abnormal isoform of prion protein in prion-infected cell lines and primary-cultured neurons by PrPSc-specific immunostaining2016

    • Author(s)
      Tanaka M, Fujiwara A, Suzuki A, Yamasaki T, Hasebe R, Masujin K, Horiuchi M
    • Journal Title

      J Gen Virol

      Volume: 97 Issue: 8 Pages: 030-2042

    • DOI

      10.1099/jgv.0.000514

    • NAID

      120006331855

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Establishment of a simple cell-based ELISA for the direct detection of abnormal isoform of prion protein from prion-infected cells without cell lysis and proteinase K treatment2016

    • Author(s)
      Shan Z, Yamasaki T, Suzuki A, Hasebe R, Horiuchi M
    • Journal Title

      Prion

      Volume: 10 Issue: 4 Pages: 305-318

    • DOI

      10.1080/19336896.2016.1189053

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Unique reactivity of cervid recombinant PrP for the detection of L-BSE and CWD by RT-QuIC2017

    • Author(s)
      Suzuki A, Yamasaki T, Hasebe R, Horiuchi M
    • Organizer
      Prion2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Therapeutic effect of autologous compact bone-derived mesenchymal stem cell transplantation on prion disease2017

    • Author(s)
      Shan Z, Hirai Y, Hayashi R, Yamasaki T, Hasebe R, Song CH, Horiuchi M
    • Organizer
      Prion2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analyses of neuron-autonomous mechanisms for neurodegeneration in prion diseases on neuron-enriched primary cell cultures2017

    • Author(s)
      Tanaka M, Yamasaki T, Suzuki A, Hasebe R, Horiuchi M
    • Organizer
      Asia Pacific Prion Symposium 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Characterization of gene expression profiles in brains of mice infected with typical and atypical BSEs2017

    • Author(s)
      Yamasaki T, Iwamaru Y, Matsuura Y, Kuroda M, Hasebe R, Okada H, Horiuchi M
    • Organizer
      Asia Pacific Prion Symposium 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Activation state of glial cells in prion diseases2017

    • Author(s)
      Kuroda M, Yamasaki T, Hasebe R, Horiuchi M
    • Organizer
      Asia Pacific Prion Symposium 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Activation state of glial cells in prion diseases2017

    • Author(s)
      Kuroda M, Yamasaki T, Hasebe R, Horiuchi M
    • Organizer
      Joint Symposium between Seoul National University-Hokkaido University “Infection and Immunity”
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Comparison of abnormal isoform of prion protein (PrPSc) in prion-infected cell lines and primary cultured neurons by PrPSc-specific immunostaining2016

    • Author(s)
      Tanaka M, Fujiwara A, Suzuki A, Yamasaki T, Hasebe R, Masujin K, Horiuchi M
    • Organizer
      Prion2016
    • Place of Presentation
      Hitotsubasi-kaikan, Tokyo, JAPAN
    • Year and Date
      2016-05-10
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Activation state of glial cells in prion diseases2016

    • Author(s)
      Horiuchi M
    • Organizer
      Prion2016
    • Place of Presentation
      Hitotsubasi-kaikan, Tokyo, JAPAN
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Neuron and glial cell type-specific detection of PrPSc in prion-infected mouse brain by flow cytometry2016

    • Author(s)
      Yamasaki T, Suzuki A, Hasebe R, Horiuchi M
    • Organizer
      Prion2016
    • Place of Presentation
      Hitotsubasi-kaikan, Tokyo, JAPAN
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Utility of cervid recombinant PrP for the detection of L-BSE and CDW prions by RT-QuIC2016

    • Author(s)
      Suzuki A, Yamasaki T, Hasebe R, Horiuchi M
    • Organizer
      第64回日本ウイルス学会
    • Place of Presentation
      札幌コンベンションセンター、札幌
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2022-02-16  

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