Analysis of collaborative activities between the capsule-positive and capsule-negative Streptococcus suis in the induction of and escape from autophagy
Project/Area Number |
16K15037
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Veterinary medical science
|
Research Institution | The University of Tokyo |
Principal Investigator |
Sekizaki Tsutomu 東京大学, 大学院農学生命科学研究科(農学部), 教授 (70355163)
|
Co-Investigator(Renkei-kenkyūsha) |
NAKAGAWA ICHIRO 京都大学, 大学院医学研究科, 教授 (70294113)
NOZAWA TAKASHI 京都大学, 大学院医学研究科, 助教 (10598858)
WATANABE TKAYASU 日本大学, 歯学部, 助教 (70725514)
|
Research Collaborator |
KUROKI KASUMI (ISHIDA KASUMI) 東京大学, 大学院農学生命科学研究科, 学振特別研究員 (80760272)
|
Project Period (FY) |
2016-04-01 – 2019-03-31
|
Project Status |
Completed (Fiscal Year 2018)
|
Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 豚レンサ球菌 / オートファジー / 莢膜 / 心内膜炎 / 感染症 / 細胞障害性 / 細胞侵入性 / 細胞壁タンパク質 / オードファジー |
Outline of Final Research Achievements |
Capsule-negative Streptococcus suis of porcine endocarditis and meningitis origins showed a higher degree of cytotoxicity and invasion to cultured cells compared with those of capsule-positive bacteria. Autophagy was observed in cultured cells expressing GFP-LC3, a biomarker of autophagosomes; however, number of invaded cells were very few and the transcriptome assay could not be performed. Twenty-two genes encoding cell-wall proteins, which were thought to play an important role in invasion, were individually deleted in the capsule-negative S. suis strain. Four deletion mutants showed lower level of biofilm formation and two of them and two other deletion mutants showed lower level of adherence to cultured cells compared with the parent strain; however, each of double-gene deletion mutants did not altered the levels of biofilm formation and adherence to cultured cells.
|
Academic Significance and Societal Importance of the Research Achievements |
Streptococcus suis無莢膜株が無莢膜株より高い細胞障害性と侵入性を示した成績は、これまで無毒と思われていた菌の新たな病原性を示した成績と言える。また、これまで全く知見がなかったStreptococcus suisにおいてもオートファジーが誘導されることが明らかになり、本菌感染症における感染初期段階での宿主応答が発症または治癒における重要な通過点であることが明らかとなった。これらの成績は、本菌感染症の予防や症状重篤化阻止に向けた新たな対策法開発にとって重要な知見となった。
|
Report
(4 results)
Research Products
(32 results)
-
-
-
-
-
-
-
-
[Journal Article] Epidemiological study on gastric Helicobacter spp. in dogs with gastrointestinal diseases in Japan: Diversity of H. heilmannii s.s.2017
Author(s)
〇Sanae Kubota-Aizawa, Koichi Ohno, Kenjiro Fukushima, Hideyuki Kanemoto, Ko Nakashima, Kazuyuki Uchida, James K. Chambers, Yuko Goto-Koshino, Takayasu Watanabe, Tsutomu Sekizaki, Hitomi Mimuro, Hajime Tsujimoto
-
Journal Title
The Veterinary Journal
Volume: 印刷中
Pages: 56-62
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
[Journal Article] A locus encoding variable defence systems against invading DNA identified in Streptococcus suis.2017
Author(s)
Okura M, Nozawa T, Watanabe T, Murase K, Nakagawa I, Takamatsu D, Osaki M, Sekizaki T, Gottschalk M, Hamada S, Maruyama F.
-
Journal Title
Genome Biol Evol
Volume: 9
Issue: 4
Pages: 1000-1012
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
[Journal Article] Epidemiological study on feline gastric <i>Helicobacter</i> spp. in Japan2017
Author(s)
〇Sanae Kubota-Aizawa, Koichi Ohno, Hideyuki Kanemoto, Ko Nakashima, Kenjiro Fukushima, Kazuyuki Uchida, James K. Chambers, Yuko Goto-Koshino, Hitomi Mimuro, Takayasu Watanabe, Tsutomu Sekizaki, Hajime Tsujimoto
-
Journal Title
Journal of Veterinary Medical Science
Volume: 79
Issue: 5
Pages: 876-880
DOI
NAID
ISSN
0916-7250, 1347-7439
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
[Journal Article] Streptococcus suis Serotype 2 Capsule In Vivo.2016
Author(s)
Jean-Philippe Auger, Nattakan Meekhanon, Masatoshi Okura, Makoto Osaki, Marcelo Gottschalk, Tsutomu Sekizaki, and Daisuke Takamatsu.
-
Journal Title
Emerg. Infect. Dis.
Volume: 22
Issue: 10
Pages: 1793-1796
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
-
-
-
-
-
-
[Presentation] Investigation of microbiota in pig farms for understanding the route of Streptococcus suis infection.2018
Author(s)
Hyunjung Kim, Sakura Arai, Kazunori Murase, Takayasu Watanabe, Kasumi Kuroki, Fumito Maruyama, Mari Tohya, Eriko Suzuki, Nachiko Takeshita, Ichiro Nakagawa, Ro Osawa, Tsutomu Sekizaki.
Organizer
第91回日本細菌学会総会 福岡国際会議場(九州大学)
Related Report
-
-
-
-
-
[Presentation] Comparison of Streptococcus suis in pig farms and the swine oral microbiota between Japan and Vietnam.2017
Author(s)
Hyunjung Kim, Sakura Arai, Takayasu Watanabe, Kazunori Murase, Fumito Maruyama, Mari Tohya, Eriko Suzuki, Nachiko Ogata,Ryoko Yamada, Shinichi Dozaki, Tan Hung Vo, Nguyen Thi Phuong Binh, Ngoc Hai Nguyen, Ichiro Nakagawa, Tsutomu Sekizaki.
Organizer
XXI LISSSD, Fiji.
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
[Presentation] Isolation and comparative genome analysis of capsule-positive and -negative Streptococcus suis from porcine endocarditis.2016
Author(s)
Tohya, M., Watanabe, T., Maruyama, F., Arai, S., Ota, A., Nakagawa, I. and Sekizaki, T.
Organizer
The 13th Korea-Japan International Symposium on Microbiology 2016
Place of Presentation
The K Hotel Gyeongju, Gyeongju city, Gyeongbuk, Korea
Year and Date
2016-05-12
Related Report
Int'l Joint Research