Comprehensive analysis of molecules expressed on the midgut of poultry red mites for the development of new anti-mite vaccines

• Project/Area Number: 16K15043
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Hokkaido University
• Principal Investigator: OHASHI Kazuhiko 北海道大学, 獣医学研究科, 教授 (90250498)
• Co-Investigator(Kenkyū-buntansha): 村田 史郎 北海道大学, 獣医学研究院, 助教 (10579163) ; 今内 覚 北海道大学, 獣医学研究院, 准教授 (40396304)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ワクモ / 中腸

Outline of Final Research Achievements

For the development of new vaccines against poultry red mite, Dermanyssus gallinae, a search was made for novel vaccine candidates expressed on the cell membrane of the midgut of the mite. Three kinds of molecules, whose expressions are predicted on the cell membrane, were identified, and their expressions on the midgut were confirmed by laser capture microdissection and RT-PCR. In addition, cathepsin D-like molecule, catD2, which was already identified in the course of the comprehensive analysis of genes expressed in the red mites done in our laboratory, was shown to be expressed in protonymph, deutonymph and adult and expressed regardless engorgement or starvation. The recombinant catD2 protein was not recognized by the sera from chickens fed by the red mites, suggesting that catD2 is a concealed antigen. Further analyses, including the detailed functions, the expression sites in red mites and the evaluation as vaccine antigens, are required to develop effective vaccines.

Research Products

• [Journal Article] Expression profile of Rhipicephalus microplus vitellogenin receptor during oogenesis (2018)
  • Author(s): Seixas A, Alzugaray MF, Tirloni L, Parizi LF, Pinto AFM, Githaka NW, Konnai S, Ohashi K, Yates JR III, Termignoni C, da Silva Vaz I Jr
  • Journal Title: Ticks and Tick-borne Diseases Volume: 9 Issue: 1 Pages: 72-81
  • DOI: 10.1016/j.ttbdis.2017.10.006
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 抗ワクモ(Dermanyssus gallinae)ワクチン開発に向けたワクモ由来フェリチン2の性状解析 (2016)
  • Author(s): 伊勢崎 政美、村田史郎、酒井英史、矢吹卓也、種子野章、市居修、伊東拓也、今内覚、大橋和彦
  • Organizer: 第159回日本獣医学会
  • Place of Presentation: 日本大学生物資源科学部、藤沢市、神奈川県
  • Year and Date: 2016-09-06