Elucidation of pathogenesis mechanism and development of diagnostic technology of Nonpathogenic pig ameba spread into Japan

• Project/Area Number: 16K15049
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Osaka Prefecture University
• Principal Investigator: Sasai Kazumi 大阪府立大学, 生命環境科学研究科, 教授 (70211935)
• Co-Investigator(Kenkyū-buntansha): 谷 浩行 大阪府立大学, 生命環境科学研究科, 准教授 (00305658) ; 松林 誠 大阪府立大学, 生命環境科学研究科, 准教授 (00321076) ; 古家 優 大阪府立大学, 生命環境科学研究科, 准教授 (30500706)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: Entamoeba / PCR / 原虫性消化器疾患 / 疫学 / 豚 / アメーバ性大腸炎 / シークエンス解析 / サブタイプ / 人と動物の共通感染症 / 原虫 / 疫学調査 / 遺伝子診断 / 下痢症 / 豚アメーバ / 病態発現機序の解明 / 診断 / 寄生虫

Outline of Final Research Achievements

Entamoeba (E) histolytica forms amebic colitis or liver abscess when infected with humans, and in severe cases it is lethal. In recent years, parasitism of Ameba-like protozoa has been confirmed in swine exhibiting a lethal gastrointestinal symptom in Japan. However, in the conventional method, the species was not clarified. In order to clarify in detail the pathology of pigs infected with E, we performed the following analysis and constructed a typing method of four subtypes. In addition, E. polecki subtypes 1 and 3 were detected in swine exhibiting domestic refractory diarrhea. From the above, it is suggested that there are three species (subtype) of E in Japan, and that E. polecki may be more widely distributed.

Research Products

• [Journal Article] Development of molecular diagnostic protocols for detecting three types of Entamoeba from diarrheal and asymptomatic pigs and environmental moist soils (2017)
  • Author(s): Yoshimasa Hirashima, Tilusha Manchanayake, Takahisa Yano, Syoei Kitahara, Terunori Koreeda, Syunsuke Kamimura, Kazumi Sasai, Makoto Matsubayashi, Tomoyuki Shibahara.
  • Journal Title: Parasitology Research Volume: 116(7) Issue: 7 Pages: 2001-2007
  • DOI: 10.1007/s00436-017-5483-1
  • Peer Reviewed / Open Access
• [Journal Article] Report of fatal mixed infection with Cryptosporidium parvum and Giardia intestinalis in neonatal calves (2017)
  • Author(s): Yuu Matsuura, Makoto Matsubayashi, Satoko Nukata, Tomoyuki Shibahara, Osamu Ayukawa, Yasuko Kondo, Tomohide Matsuo, Shigehiko Uni, Masaru Furuya, Hiroyuki Tani, Naotoshi Tsuji, Kazumi Sasai.
  • Journal Title: Acta Parasitologica Volume: 62(1) Issue: 1 Pages: 214-220
  • DOI: 10.1515/ap-2017-0026
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant