• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of a single neural circuit visualization method using a novel neurotracer virus and a tissue transparency technique.

Research Project

Project/Area Number 16K15059
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Integrative animal science
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

TANIGUCHI TAKAHIDE  東京農工大学, (連合)農学研究科(研究院), 准教授 (70282803)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsコロナウイルス / 神経回路 / コネクトーム解析 / イメージング / 発光タンパク質 / ウイルス / 蛍光タンパク質 / ニューロトレーシング
Outline of Final Research Achievements

Porcine hemagglutinating encephalomyelitis virus (PHEV) 67N strain efficiently infects rodent nerve cells. The virus strain infects adjacent neurons through synapses, and thus I conducted basic research to develop the 67 strain as a novel, safe neurotracer virus. We determined the whole genome sequence of 67N strain and found 20 amino acid substitutions in the S protein, which is important for the virus to infect neurons. Anti-N monoclonal antibodies (MAb) were produced using E. coli-expressed recombinant N protein as the antigen. I could detect PHEV-infected nerve cells and trace neuronal connection by the indirect fluorescent antibody method using these MAbs. And a highly sensitive and specific ELISA method for the detection of anti-PHEV antibodies was developed using recombinant N protein and extracted virus protein from PHEV infected cells as an antigen.

Academic Significance and Societal Importance of the Research Achievements

PHEVはコロナウイルスとしては特異な神経細胞指向性を示すウイルスである。特に67N株は齧歯類の神経細胞に感染し、シナプスを介して隣接する神経細胞に感染が進み、病原性も低いことから安全性の高い新規ニューロトレーサーウイルスとして有望である。一方、最近インフルエンザ様呼吸器感染症を引き起こすPHEVが豚から分離され、遺伝子変異による組織指向性の急変の可能性が示唆された。本研究で得られた、67N及びOSN204株のゲノム塩基配列解析データやモノクローナル抗体、新規ELISA法はPHEVのニューロトレーサーとして開発のみならず、遺伝子変異による宿主や組織指向性の変化の機構解明に役立つものと思われる。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (2 results)

All 2019 2017

All Presentation (2 results)

  • [Presentation] Development of a recombinant nucleocapsid protein based ELISA for the detection of antibodies against Porcine Hemagglutinating Encephalomyelitis (PHEV)2019

    • Author(s)
      CHAU Thi Huyen Trang, Hideki HAYASHIDANI, Takahide TANIGUCHI
    • Organizer
      第162回日本獣医学会学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 豚血球凝集性脳脊髄炎ウイルス(PHEV)高神経細胞親和性67N株と非親和性ONS204株の培養細胞での増殖性とゲノム塩基配列の比較2017

    • Author(s)
      谷口隆秀・中川美佳・堀口雅史・白濱百合
    • Organizer
      第160回 日本獣医学会学術集会 鹿児島大学
    • Related Report
      2017 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi