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Establishment of culture system of chipmunk neural stem cell for elucidating function of hibernation-specific protein (HP)

Research Project

Project/Area Number 16K15060
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Integrative animal science
Research InstitutionNiigata University

Principal Investigator

sekiima tsuneo  新潟大学, 自然科学系, 教授 (10300964)

Co-Investigator(Kenkyū-buntansha) 武井 延之  新潟大学, 脳研究所, 准教授 (70221372)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords冬眠 / シマリス / 培養 / 神経系幹細胞 / 交配実験 / 発情鳴き / 繁殖 / stem cell / neurosphere / グリア細胞 / 低温耐性 / 神経幹細胞 / HP / 幹細胞
Outline of Final Research Achievements

In this study, we aimed to generate culture cells of chipmunk neuron and to prepare a platform for elucidating molecular mechanisms of hibernation in vitro. First of all, we established the breeding system of chipmunk based on observation of chipmunk’s mating behavior to obtain chipmunk’s embryo. A female unique estrus call played a role in acceptance of male and copulation success. Through systematic mating experiments referring to female estrus call, we were able to obtain fresh chipmunk’s embryos from some pregnant females and provide them to subsequent culturing experiments. As a notable output for culture cells, we have succeeded to establish the culture system of chipmunk neural stem cells (neurosphere). These cells can be stored in LN. When necessary, cells are thawed and differentiated into mature neurons by appropriate procedures. We also make the culture device that does not require CO2. It makes low temperature culture easily without CO2 incubator.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (6 results)

All 2017 2016

All Presentation (6 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] リス科における冬眠特異的タンパク質の種間および種内変異の進化的背景2017

    • Author(s)
      鎌田泰斗、石庭寛子、 関島恒夫
    • Organizer
      日本進化学会 第19回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シマリス神経幹細胞の維持と分化2017

    • Author(s)
      武井延之
    • Organizer
      第1回冬眠休眠研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 冬眠特異的タンパク質-HP-の種間および種内比較に基づく冬眠進化プロセスの解明2017

    • Author(s)
      鎌田泰斗
    • Organizer
      第1回冬眠休眠研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 冬眠期のシマリス神経細胞におけるAMPKを上流とした翻訳の抑制2017

    • Author(s)
      山田新太郎
    • Organizer
      第1回冬眠休眠研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 冬眠期のシマリス神経細胞におけるAMPKを上流とした翻訳の抑制2016

    • Author(s)
      山田新太郎、関島恒夫、那波宏之、武井延之
    • Organizer
      第59回日本神経化学会大会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市博多区石城町2-1)
    • Year and Date
      2016-09-08
    • Related Report
      2016 Research-status Report
  • [Presentation] Intra-specific variation of hibernation style in Siberian chipmunk, Tamias sibiricus2016

    • Author(s)
      鎌田泰斗、山田新太郎、近藤宣昭、関島恒夫
    • Organizer
      The 64th NIBB Conference "Evolution of Seasonal Timers"
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県岡崎市明大寺字伝馬8-1)
    • Year and Date
      2016-04-22
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2019-03-29  

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