| Project/Area Number |
16K15060
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Integrative animal science
|
|---|
| Research Institution | Niigata University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
武井 延之 新潟大学, 脳研究所, 准教授 (70221372)
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 冬眠 / シマリス / 培養 / 神経系幹細胞 / 交配実験 / 発情鳴き / 繁殖 / stem cell / neurosphere / グリア細胞 / 低温耐性 / 神経幹細胞 / HP / 幹細胞 |
|---|
| Outline of Final Research Achievements |
In this study, we aimed to generate culture cells of chipmunk neuron and to prepare a platform for elucidating molecular mechanisms of hibernation in vitro. First of all, we established the breeding system of chipmunk based on observation of chipmunk’s mating behavior to obtain chipmunk’s embryo. A female unique estrus call played a role in acceptance of male and copulation success. Through systematic mating experiments referring to female estrus call, we were able to obtain fresh chipmunk’s embryos from some pregnant females and provide them to subsequent culturing experiments. As a notable output for culture cells, we have succeeded to establish the culture system of chipmunk neural stem cells (neurosphere). These cells can be stored in LN. When necessary, cells are thawed and differentiated into mature neurons by appropriate procedures. We also make the culture device that does not require CO2. It makes low temperature culture easily without CO2 incubator.
|
