| Project/Area Number |
16K15062
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | Nara Medical University |
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Principal Investigator |
Horie Kyoji 奈良県立医科大学, 医学部, 教授 (30333446)
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| Co-Investigator(Renkei-kenkyūsha) |
WAKAMOTO Yuichi 東京大学, 大学院総合文化研究科, 准教授 (30517884)
YOSHIDA Junko 奈良県立医科大学, 医学部, 助教 (30769196)
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| Project Period (FY) |
2016-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | ES細胞 / 遺伝子 / 1細胞 / 幹細胞 / 蛍光 / バイオテクノロジー / 蛍光蛋白 / １細胞解析 / 蛍光タンパク / 1細胞解析 |
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| Outline of Final Research Achievements |
With the advent of the next generation sequencing technologies, the single cell transcriptome analysis is making a great advance. However, the single cell transcriptome analysis lacks temporal information of gene expression in each cell. To complement this limitation, we have developed a method for long-term gene expression analysis of mouse ES cells using a microfluidic device. We have succeeded in tracking the expression of our gene of interest for one week using a fluorescent protein as a reporter. We expect the application of this technology to other cell lines.
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