Finding of silkworm monosony-why the monosomy survive-

• Project/Area Number: 16K15064
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Insect science
• Research Institution: Iwate University
• Principal Investigator: Sahara Ken 岩手大学, 農学部, 教授 (30241368)
• Co-Investigator(Kenkyū-buntansha): 金児 雄 弘前大学, 農学生命科学部, 助教 (90633610)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: モノソミー / カイコ / 第2染色体 / FISH / 形質マーカー / 定量PCR

Outline of Final Research Achievements

We find monosomy silkworm individuals which are reproducible and have both female and males. By crossing two mutant strains with the monosomy individuals we developed monosomy strain that can be discriminated by the visible phenotypes. RNAseq revealed that the expression of 2nd chromosome genes in the monosomy progenies seems to be about half amount that in the normal. Hence we concluded dosage compensation for the autosome gene cannot be functioned in the silkworms.

Research Products

• [Journal Article] Relative humidity and maintenance of p50 silkworms reared on artificial diet. (2017)
  • Author(s): Yuri K, Sahara K
  • Journal Title: Journal of Insect Biotechnology & Sericology Volume: 印刷中
  • NAID: 130005865228
  • Peer Reviewed
• [Journal Article] 幼若ホルモン生合成の制御機構 (2016)
  • Author(s): 金児 雄, 比留間 潔
  • Journal Title: 蚕糸・昆虫バイオテック Volume: 85 Pages: 117-125
  • NAID: 130006725593
  • Open Access
• [Presentation] モノソミーカイコの後代 (2018)
  • Author(s): 佐原健・松本祐希名・吉戸敦生・安河内祐二
  • Organizer: 日本蚕糸学会第88回大会
• [Presentation] 限性黄繭系統Syの染色体構成 (2017)
  • Author(s): 佐原健・吉戸敦生
  • Organizer: 日本蚕糸学会第87回大会
  • Place of Presentation: 筑波産学連携支援センター（茨城県つくば市）
  • Year and Date: 2017-03-21
• [Presentation] カイコは第2染色体1本でも生きていけるのか？ (2017)
  • Author(s): 吉戸敦生・金児雄・佐原健
  • Organizer: 日本蚕糸学会第87回大会
  • Place of Presentation: 筑波産学連携支援センター（茨城県つくば市）
  • Year and Date: 2017-03-21