Development of novel desiccation and salinity-indusible expression systems
| Project/Area Number |
16K15073
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Insect science
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
Kikawada Takahiro 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 上級研究員 (60414900)
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| Co-Investigator(Renkei-kenkyūsha) |
Gusev Oleg 国立研究開発法人理化学研究所, 予防医療・診断技術開発プログラム・研究プログラム, マネージャー (30711999)
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| Research Collaborator |
Cornette Richard
Miyata Yugo
Sogame Yoichiro
Tokumoto Shoko
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 遺伝子発現 / プロモーター / 乾燥耐性 / 昆虫 / 生体機能利用 / 遺伝子発現制御 |
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| Outline of Final Research Achievements |
Our final goal of this project is to develop a novel heterologous gene expression system based on dry- and salinity-inducible promoters in the anhydrobiotic insect, Polypedilum vanderplanki. In 2016, the promoter region for PvGapdh gene was identified as a novel constitutive promoter, which allowed effectively expressing heterologous proteins, such as GFP, in Pv11 cells, a cultured cell line derived from the anhydrobiotic insect. In 2017, we found a highly conserved motif (TCTAGAA) in the promoter regions for several desiccation-inducible genes in the insect, which motif was corresponded with Drosophila’s heat shock element (HSE). RNAi experiments demonstrated that PvHSF1, a homolog for the HSE-binding protein in the anhydrobiotic insect, could be involved in induction of the extreme desiccation tolerance in Pv11 cells.
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Report
(3 results)
Research Products
(39 results)
