Overproduction of functional membrane proteins by improvement of integration factors of E. coli
| Project/Area Number |
16K15083
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Iwate University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SHIMAMOTO Keiko 公益財団法人サントリー生命科学財団, 生物有機科学研究所, 主幹研究員 (70235638)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 膜タンパク質 / タンパク質膜挿入 / MPIase / 糖脂質酵素 / CdsA / バイオテクノロジー |
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| Outline of Final Research Achievements |
Protein integration into the cytoplasmic membranes of E. coli requires a glycolipid termed as MPIase in addition to a series of proteinaceous factors such as SRP and SecYEG. We found that CdsA that is known to be involved in phospholipid biosynthesis, is also involved in MPIase biosynthesis. CdsA is a rate-limiting enzyme for MPIase biosynthesis, since CdsA depletion causes MPIase depletion and CdsA overproduction causes MPIase overproduction. We constructed a strain, in which not only MPIase, but also SecYEG, SRP and YidC were overproduced. By means of this strain, we achieved overproduction of several membrane proteins including F0c. F0c.
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Report
(3 results)
Research Products
(43 results)
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[Presentation] CdsA is involved in biosynthesis of MPIase essential for membrane protein integration in vivo2018
Author(s)
Sawasato, K., Sato, R., Nishikawa, H., Iimura, N., Kamemoto, Y., Fujikawa, K., Yamaguchi, T., Kuruma, Y., Tamura, Y., Endo, T., Ueda, T., Shimamoto, K., Nishiyama, K.
Organizer
Gordon Research Conference on Protein Transport Across Cell Membranes. (Hotel Galvez, Galveston, TX, USA)
Related Report
Int'l Joint Research
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