Approach to identify a novel mechanism which modulates vitaminA regulation of adipocyte differentiation

• Project/Area Number: 16K15090
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Kyoto University
• Principal Investigator: Kioka Noriyuki 京都大学, 農学研究科, 准教授 (90234179)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ビタミンA / 脂肪細胞 / 細胞外マトリックス / 間葉系幹細胞 / コラーゲン / レチノイン酸 / 分化

Outline of Final Research Achievements

Differentiation of mesenchymal stem cells can be regulated by either retinoic acid, an active metabolite of vitamin A, or stiffness of extracellular matrix (ECM). We previously reported that vinexin, a focal adhesion protein, is involved in sensing ECM stiffness. Given the association of vinexin with retinoic acid receptor, it is hypothesized that retinoic acid and ECM stiffness function cooperatively for regulating the differentiation of mesenchymal stem cells. We examined the effect of vinexin depletion on retinoic acid-induced differentiation. The result partly supports the hypothesis, but also suggests complicated regulation.

Research Products

• [Int'l Joint Research] University of Michigan(米国)
• [Journal Article] Vinculin promotes nuclear localization of TAZ to inhibit ECM stiffness-dependent differentiation into adipocytes (2017)
  • Author(s): Mito Kuroda, Hiroki Wada, Yasuhisa Kimura, Kazumitsu Ueda and Noriyuki Kioka
  • Journal Title: Journal of Cell Science Volume: 130 Issue: 5 Pages: 989-1002
  • DOI: 10.1242/jcs.194779
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The distribution of vinculin to lipid rafts plays an important role in sensing stiffness of extracellular matrix (2017)
  • Author(s): Ayaka Ichikawa Nagasato, Hiroshi Yamashita, Michinori Matsuo, Kazumitsu Ueda and Noriyuki Kioka
  • Journal Title: Bioscience Biotechnology Biochemistry Volume: 印刷中 Issue: 6 Pages: 1136-1147
  • DOI: 10.1080/09168451.2017.1289074
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Vinculin Association with Actin Cytoskeleton is Necessary for Stiffness-dependent Regulation of Vinculin Behavior. (2017)
  • Author(s): Tomohiro Omachi, Takafumi Ichikawa, Yasuhisa Kimura, Kazumitsu Ueda, and Noriyuki Kioka.
  • Journal Title: PLos One Volume: 12 Issue: 4 Pages: e0175324-e0175324
  • DOI: 10.1371/journal.pone.0175324
  • NAID: 120006309292
  • Peer Reviewed / Open Access
• [Journal Article] Vinexin family (SORBS) proteins play different roles in stiffness-sensing and contractile force generation (2017)
  • Author(s): Ichikawa, T., Kita, M., Matsui, T.S., Ichikawa-Nagasato, A., Araki, T., Chiang, S.H., Sezaki, T., Kimura, Y., Ueda, K., Deguchi, S., Saltiel, A.R., Kioka, N.
  • Journal Title: Journal of Cell Science Volume: 130 Issue: 20 Pages: 3517-3531
  • DOI: 10.1242/jcs.200691
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Fluid shear stress applied by orbital shaking induces MG-63 osteosarcoma cells to activate ERK in two phases through distinct signaling pathways (2017)
  • Author(s): Fukada Takashi、Sakajiri Hiroki、Kuroda Mito、Kioka Noriyuki、Sugimoto Kenji
  • Journal Title: Biochemistry and Biophysics Reports Volume: 9 Pages: 257-265
  • DOI: 10.1016/j.bbrep.2017.01.004
  • Peer Reviewed
• [Journal Article] Lysophosphatidylcholine export by human ABCA7 (2017)
  • Author(s): Maiko Tomioka, Yoshinobu Toda, Noralyn B. Manucat, Hiroyasu Akatsu, Manabu Fukumoto, Nozomu Kono, Hiroyuki Arai, Noriyuki Kioka, and Kazumitsu Ueda
  • Journal Title: Biochimica Biophysica Acta Volume: 印刷中 Issue: 7 Pages: 658-665
  • DOI: 10.1016/j.bbalip.2017.03.012
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] ABCG1 and ABCG4 Suppress γ-Secretase Activity and Amyloid β Production (2016)
  • Author(s): Osamu Sano, Maki Tsujita, Yuuji Shimizu, Reiko Kato, Aya Kobayashi, Noriyuki Kioka, Alan T Remaley, Makoto Michikawa, Kazumitsu Ueda, and Michinori Matsuo
  • Journal Title: PLoS One Volume: 11 Issue: 5 Pages: e0155400-e0155400
  • DOI: 10.1371/journal.pone.0155400
  • NAID: 120005997762
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 間葉系幹細胞の細胞外基質の硬さに対する応答におけるSORBSファミリータンパク質の役割 (2018)
  • Author(s): 黒田美都, 植田和光, 木岡紀幸
  • Organizer: 日本農芸化学会 2018年度大会
• [Presentation] 超音波処理を用いた接着斑単離法の確立 (2018)
  • Author(s): 柴原正和、市川尚文、箕浦広大、木村泰久、植田和光、木岡紀幸
  • Organizer: 日本農芸化学会 2018年度大会
• [Presentation] 間葉系幹細胞分化のレチノイン酸制御に与える接着斑タンパク質ビネキシンの影響 (2017)
  • Author(s): 高田 恭子,黒田 美都, 木村 泰久,植田 和光, 木岡 紀幸
  • Organizer: 2017年(平成30年度)日本農芸化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2017-03-17
• [Presentation] ビネキシンのホスファチジン酸結合領域の解析 (2017)
  • Author(s): 伊藤 有亮. 、市川 尚文、折田 雄一郎、安部 真人、木村 泰久、植田 和光、三芳 秀人、木岡 紀幸
  • Organizer: 2017年(平成29年度)日本農芸化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2017-03-17
• [Presentation] Vinculin Promotes Nuclear Localization of TAZ to Inhibit ECM Stiffness-Dependent Differentiation into Adipocytes (2017)
  • Author(s): Mito Kuroda, Hiroki Wada, Yasuhisa Kimura, Kazumitsu Ueda, and Noriyuki Kioka
  • Organizer: Gorden Research Conference on Fibronectin, Integrin & Related Molecules
  • Place of Presentation: Ventura, CA 米国
  • Year and Date: 2017-01-29
  • Int'l Joint Research
• [Presentation] 細胞外基質の硬さの感知と収縮力発生におけるビネキシンファミリータンパク質の役割 (2017)
  • Author(s): 市川尚文、喜多真弘、植田和光、〇木岡紀幸
  • Organizer: 第69回日本細胞生物学会大会
• [Presentation] ビンキュリンは細胞外基質の硬さに応じてTAZの核局在を促進し、脂肪細胞への分化を抑制する (2017)
  • Author(s): 黒田美都, 和田洋樹, 植田和光, 木岡紀幸
  • Organizer: 第69回日本細胞生物学会大会
• [Presentation] The Distribution of Vinculin to Lipid Rafts Plays an Important Role in Sensing Stiffness of Extracellular Matrix (2017)
  • Author(s): Ayaka Nagasato-Ichikawa, Hiroshi Yamashita, Michinori Matsuo, Kazumitsu Ueda, and Noriyuki Kioka
  • Organizer: Mechanical forces in Biology
  • Int'l Joint Research
• [Presentation] 間葉系幹細胞の脂肪細胞分化におけるビネキシンファミリータンパク質の役割 (2017)
  • Author(s): 黒田美都, 植田和光, 木岡紀幸
  • Organizer: 第22回アディポサイエンス・シンポジウム
• [Presentation] 細胞外マトリックスの硬さによる細胞分化制御へのビンキュリンーYAP/TAZ系の関与 (2017)
  • Author(s): 黒田美都、市川尚文、長里彩花、大町朋弘、木村泰久、植田和光、〇木岡紀幸
  • Organizer: 2017年度生命科学系学会合同年次大会
  • Invited
• [Presentation] 接着斑タンパク質ビネキシンおよびCAPはYAP/TAZの核局在を調節する (2017)
  • Author(s): 黒田美都, 植田和光, 木岡紀幸
  • Organizer: 2017年度生命科学系学会合同年次大会
• [Presentation] 接着斑タンパク質ビネキシンがレチノイン酸により制御される間葉系幹細胞分化に与える効果 (2017)
  • Author(s): 高田恭子、黒田美都、木村泰久、植田和光、木岡紀幸
  • Organizer: 2017年度生命科学系学会合同年次大会
• [Presentation] VINCULIN AND VINEXIN FAMILY (SORBS) PROTEINS IN MECHANOSENSING AND MECHANOTRANSDUCTION (2017)
  • Author(s): Noriyuki Kioka, Takafumi Ichikawa, Mito Kuroda, Yasuhisa Kimura, Kazumitsu Ueda
  • Organizer: 3RD INTERNATIONAL SYMPOSIUM ON MECHANOBIOLOGY
  • Int'l Joint Research / Invited
• [Presentation] 細胞非接着能を有するフッ素改質基材の開発 (2016)
  • Author(s): 小泉美子、久保田浩治、森田正道、木岡紀幸、植田和光
  • Organizer: 第65回高分子討論会
  • Place of Presentation: 横浜
  • Year and Date: 2016-09-14
• [Presentation] Focal Adhesion Protein Vinculin Regulates ECM-Stiffness Dependent Differentiation to Adipocytes through Promoting Nuclear Localization of YAP/TAZ (2016)
  • Author(s): Mito Kuroda, Hiroki Wada, Kazumitsu Ueda, and Noriyuki Kioka
  • Organizer: 12th International Congress on Cell Biology (ICCB2016)
  • Place of Presentation: チェコ プラハ
  • Year and Date: 2016-07-21
  • Int'l Joint Research
• [Remarks] 京都大学農学研究科細胞生化学研究室
  • URL: http://www.biochemistry.kais.kyoto-u.ac.jp/
• [Remarks] 細胞生化学研究室
  • URL: http://www.biochemistry.kais.kyoto-u.ac.jp/