| Project/Area Number |
16K15091
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | エマルジョンドロップレット / 微小培養 / 環境微生物 / 難培養性微生物 / ミリオンスケール / エマルジョンドロップ / 培養工学 |
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| Outline of Final Research Achievements |
We examined the application possibility of emulsion drop culture system for environmental BNC (viable but non-culturable) microbiome. First, we established the method to produce the emulsion drop stably and then showed the growth of E. coli and yeast in emulsion drop. Next, when the microbiome flora in the emulsion drop in which the environmental soil microbiome were enclosed were analyzed by 16S rDNA NGS analysis, the growth of environmental BNC (viable but non-culturable) microbiome were detected. This indicated the emulsion drop system is new technology for analyzing the environmental microbiome.
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