| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Recently, a certain populations of human dendritic cells (DCs) in peripheral blood were identified as FcepsilonRI-expressing cells, in addition to classically well-known FcepsilonRI-expressing cells, mast cells and basophils. In contrast, mouse DCs do not express FcepsilonRI on cell surface. Therefore, the physiological role of FcepsilonRI on DCs is largely unknown. This background prompted me to generate mutant mouse possessing FcepsilonRI-expressing DCs. To obtain the mutant FcepsilonRI alpha, which can be expressed on mouse DCs and exhibits high affinity to mouse IgE, we introduced several amino acid substitutions on IgE-binding region of human FcepsilonRI alpha, considering structure-function relationship. Finally, we found that the human FcepsilonRI alpha mutant, in which 4 amino acids were replaced to mouse type residues, was expressed on cell surface of mouse DCs with endogenous FcepsilonRI gannma and bound mouse IgE with high affinity as that of mouse FcepsilonRI alpha.
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