Construction of mutant FcepsilonRIalpha to generate mutant mouse possessing FcepsilonRI-expressing dendritic cells
| Project/Area Number |
16K15092
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | IgE受容体 / FcεRI / 樹状細胞 / IgE抗体 / マスト細胞 / 好塩基球 / 変異体 / アレルギー / 免疫応答 |
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| Outline of Final Research Achievements |
Recently, a certain populations of human dendritic cells (DCs) in peripheral blood were identified as FcepsilonRI-expressing cells, in addition to classically well-known FcepsilonRI-expressing cells, mast cells and basophils. In contrast, mouse DCs do not express FcepsilonRI on cell surface. Therefore, the physiological role of FcepsilonRI on DCs is largely unknown. This background prompted me to generate mutant mouse possessing FcepsilonRI-expressing DCs. To obtain the mutant FcepsilonRI alpha, which can be expressed on mouse DCs and exhibits high affinity to mouse IgE, we introduced several amino acid substitutions on IgE-binding region of human FcepsilonRI alpha, considering structure-function relationship. Finally, we found that the human FcepsilonRI alpha mutant, in which 4 amino acids were replaced to mouse type residues, was expressed on cell surface of mouse DCs with endogenous FcepsilonRI gannma and bound mouse IgE with high affinity as that of mouse FcepsilonRI alpha.
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Report
(3 results)
Research Products
(59 results)
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[Journal Article] Role of PU.1 expression as an inflammatory marker in experimental autoimmune uveoretinitis.2017
Author(s)
Umazume, A., Kezuka, T., Matsuda, R., Usui, Y., Takahashi, H., Yamakawa, N., Yashiro, T., Nishiyama, C., and Goto, H.
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Journal Title
Ocular Immunology and Inflammation
Volume: 印刷中
Related Report
Peer Reviewed
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[Journal Article] The hematopoietic cell-specific transcription factor PU.1 is critical for expression of CD11c.2017
Author(s)
Yashiro T, Kasakura K, Oda Y, Kitamura N, Inoue A, Nakamura S, Yokoyama H, Fukuyama K, Hara M, Ogawa H, Okumura K, Nishoiyama M, Nishoiyama C
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Journal Title
International immunology
Volume: 29
Issue: 2
Pages: 87-94
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Pharmacological inhibition of Notch signaling suppresses food antigen-induced mucosal mast cell hyperplasia.2017
Author(s)
Honjo A, Nakano N, Yamazaki S, Hara M, Uchida K, Kitaura J, Nishiyama C, Yagita H, Ohtsuka Y, Ogawa H, Okumura K, Shimizu T
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Journal Title
J Allergy Clin Immunol.
Volume: 139
Issue: 3
Pages: 987-996
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Transcription factor Tlx1 regulates the ability of spleen mechenchymal stromal cells to support the survival of hematopoietic progenitor cells in vitro.2016
Author(s)
Tezuka, T., Kasahara, T., Ueno, Y., Nishiyama, C., Oda, A., Goitsuka, R.
Organizer
The 45th Annual Meeting of The Japanese Society for Immunology
Place of Presentation
沖縄
Year and Date
2016-12-05
Related Report
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[Presentation] Interdependent roles of Tlx1-expressing mesenchymal cells and macrophages in extra medullary hematopoiesis in the spleen.2016
Author(s)
Oda, A., Tezuka, T., Kasahara, T., Ueno, Y., Nishiyama, C., Goitsuka, R.
Organizer
The 45th Annual Meeting of The Japanese Society for Immunology
Place of Presentation
沖縄
Year and Date
2016-12-05
Related Report
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