Shotgun proteomics beyond trypsin
| Project/Area Number |
16K15107
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SUGIYAMA Naoyuki 京都大学, 大学院薬学研究科, 准教授 (50545704)
WAKABAYASHI Masaki 京都大学, 大学院薬学研究科, 連携准教授 (70552024)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | ショットガンプロテオミクス / トリプシン / トリプエヌ / タンパク質末端解析 / 分析化学 / プロテオミクス / 質量分析 |
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| Outline of Final Research Achievements |
We investigated the usefulness of various proteases for shotgun proteomics, and found that TrypN, belonging to LysArginase family, has quite unique features. We optimized the digestion conditions as well as the separation conditions for tryp-N digested peptides. As a result, we found that tryp-N peptides can be separated based on the positive charged positions on peptides by strong cation exchange chromatography. Using the separation selectivity, we successfully enrich the protein N-terminal peptides, applying to N-terminomics.
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Report
(3 results)
Research Products
(17 results)
