| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
To elucidate the mysteries of hibernation of mammals whose molecular mechanism is still unknown, we aimed to produce transgenic Syrian hamsters useful for hibernation research by applying recently developed genome editing technology to a mammalian hibernator, Syrian hamsters. Because it is known that oocyte of Syrian hamsters is vulnerable to light, we attempted to apply the GONAD method, a recently developed method which introduces Cas9 complexes into oviducts by electroporation. We succeeded in introducing the nucleic acid itself into the fertilized egg, but failed to obtain genetically modified animals. The reasons for this is unclear. GONAD conditions for mouse or our breeding environments in the same room with mice might not be suitable for Syrian hamsters. We will improve these points in future.
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