Screening for functional protein-encoding ORFs located outside coding regions in the human genome
| Project/Area Number |
16K15116
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
Aizawa Yasunori 東京工業大学, バイオ研究基盤支援総合センター, 講師 (90418674)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKAO KEIZO 富山大学, 研究推進機構研究推進総合支援センター, 教授 (80420397)
TAGUCHI HIDEKI 東京工業大学, 生命理工学院, 教授 (40272710)
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| Research Collaborator |
YAMASHITA HITOYOSHI
SASAKI HIROTAKA
KURASAWA HIKARU
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 非コードゲノム / ORF / ミトコンドリア / 膜電位 / ペプチドモチーフ / 翻訳 / 上流ORF / ノンコードRNA / 遺伝子 / プロテオーム |
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| Outline of Final Research Achievements |
This study identified a novel candidate of small ORFs (uORF13) that is located within untranslated regions of human messenger RNAs and encodes functional proteins. The uORF13-encoding protein (uORF13p) is localized at mitochondria and seems to be involved in regulation of the membrane potential. Alanine-scanning mutagenesis allowed us to find two different peptide motifs, which can have opposite roles in the membrane potential control. In addition to our first example of functional protein-encoding small ORF located in noncoding regions, this finding also supports that the human genome encodes more functional proteins which we have yet to discover.
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Report
(3 results)
Research Products
(5 results)
