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Genetic tools for circuit-based drug discovery

Research Project

Project/Area Number 16K15125
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Pharmacology in pharmacy
Research InstitutionKyoto University

Principal Investigator

Nagayasu Kazuki  京都大学, 薬学研究科, 助教 (00717902)

Co-Investigator(Renkei-kenkyūsha) KANEKO Shuji  京都大学, 大学院薬学研究科, 教授 (60177516)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsウイルスベクター / セロトニン / 光遺伝学
Outline of Final Research Achievements

Neural transmission is achieved through three steps of biological processes. Depolarization and firing at cell somata, propagation of action potential, and release of neurotransmitters from nerve terminals. Different from other types of neurons, serotonin neurons and dopamine neurons release their transmitters from cell somata. However, physiological significance of this type of transmitter release is still unknown. In this research, we established viral-vector based platform for expressing miniSOG, a genetic tool for photo-inducible organelle inactivator, specifically in serotonin neurons.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (36 results)

All 2018 2017 2016 Other

All Int'l Joint Research (2 results) Journal Article (15 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 15 results,  Open Access: 10 results,  Acknowledgement Compliant: 3 results) Presentation (18 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Remarks (1 results)

  • [Int'l Joint Research] ブリストル大学(英国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] ブリストル大学(英国)

    • Related Report
      2016 Research-status Report
  • [Journal Article] TRPM2 Channel Aggravates CNS Inflammation and Cognitive Impairment via Activation of Microglia in Chronic Cerebral Hypoperfusion2018

    • Author(s)
      Miyanohara Jun、Kakae Masashi、Nagayasu Kazuki、Nakagawa Takayuki、Mori Yasuo、Arai Ken、Shirakawa Hisashi、Kaneko Shuji
    • Journal Title

      The Journal of Neuroscience

      Volume: 38 Issue: 14 Pages: 3520-3533

    • DOI

      10.1523/jneurosci.2451-17.2018

    • NAID

      120006630983

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Ketamine-Induced Prefrontal Serotonin Release Is Mediated by Cholinergic Neurons in the Pedunculopontine Tegmental Nucleus2018

    • Author(s)
      Kinoshita Haruko、Nishitani Naoya、Nagai Yuma、Andoh Chihiro、Asaoka Nozomi、Kawai Hiroyuki、Shibui Norihiro、Nagayasu Kazuki、Shirakawa Hisashi、Nakagawa Takayuki、Kaneko Shuji
    • Journal Title

      Int J Neuropsychopharmacol.

      Volume: 21 Issue: 3 Pages: 305-310

    • DOI

      10.1093/ijnp/pyy007

    • NAID

      120006469165

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Elucidation of the Role of Dorsal Raphe Serotonergic Neurons in Mood Regulation Using Pharmacological and Viral Vector-based Approaches2017

    • Author(s)
      Nagayasu K.
    • Journal Title

      YAKUGAKU ZASSHI

      Volume: 137 Issue: 3 Pages: 341-346

    • DOI

      10.1248/yakushi.16-00240

    • NAID

      130005396835

    • ISSN
      0031-6903, 1347-5231
    • Year and Date
      2017-03-01
    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Chronic antidepressant potentiates spontaneous activity of dorsal raphe serotonergic neurons by decreasing GABAB receptor-mediated inhibition of L-type calcium channels2017

    • Author(s)
      Asaoka Nozomi、Nishitani Naoya、Kinoshita Haruko、Kawai Hiroyuki、Shibui Norihiro、Nagayasu Kazuki、Shirakawa Hisashi、Nakagawa Takayuki、Kaneko Shuji
    • Journal Title

      Sci Rep

      Volume: 7 Issue: 1 Pages: 13609-13609

    • DOI

      10.1038/s41598-017-13599-3

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] High-speed and scalable whole-brain imaging in rodents and primates2017

    • Author(s)
      Seiriki K, Kasai A, Hashimoto T, Schulze W, Niu M, Yamaguchi S, Nakazawa T, Inoue K, Uezono S, Takada M, Naka Y, Igarashi H, Tanuma M, Waschek JA, Ago Y, Tanaka KF, Hayata-Takano A, Nagayasu K, Shintani N, Hashimoto R, Kunii Y, Hino M, Matsumoto J, Yabe H, Nagai T, Fujita K, Matsuda T, Takuma K, Baba A, Hashimoto H
    • Journal Title

      Neuron

      Volume: 94 Issue: 6 Pages: 1085-1100

    • DOI

      10.1016/j.neuron.2017.05.017

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Sphingosine-1-phosphate induces Ca2+ signaling and CXCL1 release via TRPC6 channel in astrocytes.2017

    • Author(s)
      Shirakawa H, Katsumoto R, Iida S, Miyake T, Higuchi T, Nagashima T, Nagayasu K, Nakagawa T, Kaneko S
    • Journal Title

      Glia

      Volume: 65 Issue: 6 Pages: 1005-1016

    • DOI

      10.1002/glia.23141

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Colchicine alleviates acute postoperative pain but delays wound repair in mice: Roles of neutrophils and macrophages2017

    • Author(s)
      Sukeishi Asami、Isami Koichi、Hiyama Haruka、Imai Satoshi、Nagayasu Kazuki、Shirakawa Hisashi、Nakagawa Takayuki、Kaneko Shuji
    • Journal Title

      Molecular Pain

      Volume: 13 Pages: 1-12

    • DOI

      10.1177/1744806917743680

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Distinct Mechanism of Cysteine Oxidation-Dependent Activation and Cold Sensitization of Human Transient Receptor Potential Ankyrin 1 Channel by High and Low Oxaliplatin2017

    • Author(s)
      Miyake Takahito、Nakamura Saki、Meng Zhao、Hamano Satoshi、Inoue Keisuke、Numata Tomohiro、Takahashi Nobuaki、Nagayasu Kazuki、Shirakawa Hisashi、Mori Yasuo、Nakagawa Takayuki、Kaneko Shuji
    • Journal Title

      Frontiers in Physiology

      Volume: 8 Pages: 878-878

    • DOI

      10.3389/fphys.2017.00878

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Pathophysiological Role of Transient Receptor Potential Ankyrin 1 in a Mouse Long-Lasting Cystitis Model Induced by an Intravesical Injection of Hydrogen Peroxide2017

    • Author(s)
      Oyama Shohei、Dogishi Koji、Kodera Mizuki、Kakae Masashi、Nagayasu Kazuki、Shirakawa Hisashi、Nakagawa Takayuki、Kaneko Shuji
    • Journal Title

      Frontiers in Physiology

      Volume: 8 Pages: 877-877

    • DOI

      10.3389/fphys.2017.00877

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pharmacological approach for elucidating the role of dorsal raphe serotonin neurons in mood/emotion regulation2017

    • Author(s)
      Nagayasu K, Kaneko S.
    • Journal Title

      Folia Pharmacologica Japonica

      Volume: 149 Issue: 1 Pages: 44-47

    • DOI

      10.1254/fpj.149.44

    • NAID

      130005188226

    • ISSN
      0015-5691, 1347-8397
    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Differential gene expression profiles in neurons generated from lymphoblastoid B-cell line-derived iPS cells from monozygotic twin cases with treatment-resistant schizophrenia and discordant responses to clozapine.2017

    • Author(s)
      Nakazawa T, Kikuchi M, Ishikawa M, Yamamori H, Nagayasu K, Matsumoto T, Fujimoto M, Yasuda Y, Fujiwara M, Okada S, Matsumura K, Kasai A, Hayata-Takano A, Shintani N, Numata S, Takuma K, Akamatsu W, Okano H, Nakaya A, Hashimoto H, Hashimoto R.
    • Journal Title

      Schizophr Res.

      Volume: 181 Pages: 75-82

    • DOI

      10.1016/j.schres.2016.10.012

    • NAID

      120007037467

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] セロトニン神経機能の光/化学遺伝学的解析の現状2017

    • Author(s)
      永安 一樹
    • Journal Title

      精神科

      Volume: 30 Pages: 105-110

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] De novo POGZ mutations in sporadic autism disrupt the DNA-binding activity of POGZ2016

    • Author(s)
      Matsumura K, Nakazawa T, Nagayasu K, Gotoda-Nishimura N, Kasai A, Hayata-Takano A, Shintani N, Yamamori H, Yasuda Y, Hashimoto R, Hashimoto H.
    • Journal Title

      J. Mol. Psychiatry

      Volume: 4 Issue: 1 Pages: 1-1

    • DOI

      10.1186/s40303-016-0016-x

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Prostaglandin D2 signaling mediated by the CRTH2 receptor is involved in MK-801-induced cognitive dysfunction.2016

    • Author(s)
      Onaka Y, Shintani N, Nakazawa T, Kanoh T, Ago Y, Matsuda T, Hashimoto R, Ohi K, Hirai H, Nagata KY, Nakamura M, Kasai A, Hayata-Takano A, Nagayasu K, Takuma K, Ogawa A, Baba A, Hashimoto H.
    • Journal Title

      Behav Brain Res.

      Volume: 314 Pages: 477-486

    • DOI

      10.1016/j.bbr.2016.07.050

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A new designer drug 5F-ADB activates midbrain dopaminergic neurons but not serotonergic neurons.2016

    • Author(s)
      Asaoka N, Kawai H, Nishitani N, Kinoshita H, Shibui N, Nagayasu K, Shirakawa H, Kaneko S.
    • Journal Title

      J Toxicol Sci.

      Volume: 41 Pages: 813-816

    • NAID

      130005281915

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] Development of the viral vector capable of optogenetic control of serotonergic neurons in rat2017

    • Author(s)
      Naoya Nishitani, Nozomi Asaoka, Haruko Kinoshita, Hiroyuki Kawai, Norihiro Shibui, Kazuki Nagayasu, Hisashi Shirakawa, Takayuki Nakagawa, Shuji Kaneko
    • Organizer
      第90回薬理学会年会
    • Place of Presentation
      長崎県
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Chronic antidepressant treatment increases pacemaker activity of dorsal raphe serotonergic neurons2017

    • Author(s)
      Nozomi Asaoka, Naoya Nishitani, Hiroyuki Kawai, Haruko Kinoshita, Norihiro Shibui, Kazuki Nagayasu, Hisashi Shirakawa, Takayuki Nakagawa, Shuji Kaneko
    • Organizer
      第90回薬理学会年会
    • Place of Presentation
      長崎県
    • Year and Date
      2017-03-15
    • Related Report
      2016 Research-status Report
  • [Presentation] Chronic treatment with a selective serotonin reuptake inhibitor increases spontaneous activity of dorsal raphe nucleus serotonergic neurons through activating L-type voltage dependent Ca2+ channels2017

    • Author(s)
      Nozomi Asaoka1, Naoya Nishitani1, Haruko Kinoshita1, Hiroyuki Kawai1, Norihiro Shibui1, Kazuki Nagayasu1, Hisashi Shirakawa1, Takayuki Nakagawa2, Shuji Kaneko1
    • Organizer
      Neuroscience2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 新規強迫性障害モデル評価系の作成:妥当性評価から治療ターゲット探索まで2017

    • Author(s)
      浅岡希美1、西谷直也1、永安一樹1、白川久志1、中川貴之2、金子周司1
    • Organizer
      第132回 日本薬理学会近畿部会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ミクログリアにおいてTRPV4の開口はLPS誘発IL10産生を促進する2017

    • Author(s)
      富澤恵里、三宅崇仁、永安一樹、白川久志、金子周司
    • Organizer
      第132回 日本薬理学会近畿部会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ケタミンの前頭前野セロトニン遊離作用におけるアセチルコリン神経核の関与2017

    • Author(s)
      木ノ下晴子1、西谷直也1、浅岡希美1、河合洋幸1、澁井紀宏1、永井佑茉1、安藤千紘1、永安一樹1、白川久志1、中川貴之2、金子周司1
    • Organizer
      第132回 日本薬理学会近畿部会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ゲノム編集技術を用いた脳神経核特異的セロトニン受容体欠損マウス作製の試み2017

    • Author(s)
      澁井紀宏1、西谷直也1、永井佑茉1、安藤千紘1、浅岡希美1、河合洋幸1、木ノ下晴子1、白川久志1、中川貴之2、大村優3、永安一樹1、金子周司1
    • Organizer
      第67回 日本薬学会近畿支部総会・大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] TRPM2を介したケモカインCXCL2産生が多発性硬化症の増悪に関与する2017

    • Author(s)
      平瀬僚、筒井真人、永安一樹、白川久志、金子周司
    • Organizer
      第131回 日本薬理学会近畿部会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 過酸化水素誘発膀胱炎モデルにおけるTRPA1の病態生理学的役割2016

    • Author(s)
      尾山 翔平, 土岸 広治, 小寺 みずき, 永安 一樹, 白川 久志, 中川 貴之, 金子 周司
    • Organizer
      第130回日本薬理学会近畿部会
    • Place of Presentation
      京都府
    • Year and Date
      2016-11-19
    • Related Report
      2016 Research-status Report
  • [Presentation] 危険ドラッグ5F-ADB のドパミン・セロトニン神経活動に対する作用2016

    • Author(s)
      浅岡 希美, 河合 洋幸, 西谷 直也, 木ノ下 晴子, 澁井 紀宏, 永安 一樹, 白川 久志, 金子 周司
    • Organizer
      第130回日本薬理学会近畿部会
    • Place of Presentation
      京都府
    • Year and Date
      2016-11-19
    • Related Report
      2016 Research-status Report
  • [Presentation] オリゴデンドロサイト前駆細胞の増殖および分化におけるTRPV4 の 生理的役割2016

    • Author(s)
      出屋敷 綾音, 三宅 崇仁, 永安 一樹, 白川 久志, 中川 貴之, 金子 周司
    • Organizer
      第130回日本薬理学会近畿部会
    • Place of Presentation
      京都府
    • Year and Date
      2016-11-19
    • Related Report
      2016 Research-status Report
  • [Presentation] 糖尿病性神経障害モデルにおける感覚異常の病態解析とTRPA1の関与2016

    • Author(s)
      緋山遥、宗可奈子、矢野佑一、永安一樹、白川久志、中川貴之、金子周司
    • Organizer
      第66回日本薬学会近畿支部総会・大会
    • Place of Presentation
      大阪府
    • Year and Date
      2016-10-15
    • Related Report
      2016 Research-status Report
  • [Presentation] マウス多発性硬化症モデルにおけるTRPM2 の病態生理学的役割2016

    • Author(s)
      平瀬 僚、筒井真人、永安一樹、白川久志、 中川貴之、金子周司
    • Organizer
      第66回日本薬学会近畿支部総会・大会
    • Place of Presentation
      大阪府
    • Year and Date
      2016-10-15
    • Related Report
      2016 Research-status Report
  • [Presentation] 培養後根神経節ニューロンにおけるノイロトロピンの作用解析2016

    • Author(s)
      河合洋幸、浅岡希美、三宅崇仁、永安一樹、白川久志、 今井哲司、中川貴之、金子周司
    • Organizer
      第66回日本薬学会近畿支部総会・大会
    • Place of Presentation
      大阪府
    • Year and Date
      2016-10-15
    • Related Report
      2016 Research-status Report
  • [Presentation] マウスミクログリアの貪食におけるTRPV チャネルの機能解析2016

    • Author(s)
      富澤恵里、三宅崇仁、永安一樹、白川久志、 中川貴之、金子周司
    • Organizer
      第66回日本薬学会近畿支部総会・大会
    • Place of Presentation
      大阪府
    • Year and Date
      2016-10-15
    • Related Report
      2016 Research-status Report
  • [Presentation] ケタミンの前頭前野セロトニン遊離作用におけるコリン作動性神経の関与2016

    • Author(s)
      ○木ノ下晴子、西谷直也、永安一樹、浅岡希美、 河合洋幸、澁井紀宏、白川久志、中川貴之、金子周司
    • Organizer
      第66回日本薬学会近畿支部総会・大会
    • Place of Presentation
      大阪府
    • Year and Date
      2016-10-15
    • Related Report
      2016 Research-status Report
  • [Presentation] 細胞種特異的レンチウイルスベクターを用いたセロトニン神経機能の光遺伝学的解析2016

    • Author(s)
      永安一樹
    • Organizer
      第66回日本薬学会近畿支部総会・大会
    • Place of Presentation
      大阪府
    • Year and Date
      2016-10-15
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 神経障害性疼痛および術後痛の病態形成に好中球の早期浸潤が与える影響2016

    • Author(s)
      助石有沙美, 勇昂一, 今井哲司, 永安一樹, 白川久志, 中川貴之, 金子周司
    • Organizer
      第129回日本薬理学会近畿部会
    • Place of Presentation
      広島県
    • Year and Date
      2016-06-24
    • Related Report
      2016 Research-status Report
  • [Remarks] 分野ホームページ

    • URL

      http://www.pharm.kyoto-u.ac.jp/channel/

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2022-02-21  

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