| Project/Area Number |
16K15131
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Natural medicines
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
田中 宏幸 九州大学, 薬学研究院, 准教授 (30253470)
坂元 政一 九州大学, 薬学研究院, 助教 (50610177)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | パーオキシダーゼ / コガネバナ / ケシ / 二次代謝産物 / モノクローナル抗体 / モルヒネ / 中和抗体 / 代謝酵素 / 抗体 / single-chain FV |
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| Outline of Final Research Achievements |
To develop the medicinal plants containing large amounts of useful metabolites, in this study I attempted to increase the contents of medicinally-important compounds in the plants by inhibiting the activity of metabolic enzyme through the expression of their neutralizing antibodies.
I first prepared the antigen Scutellaria peroxidases 1 and 2 which metabolite baicalein in Scutellaria baicalensis. Scutellaria peroxidases 1 and 2 were expressed in E.coli. Because the recombinant Scutellaria peroxidases 1 and 2 lacked the enzyme activity, refolding of the recombinant proteins was conducted.When the recombinant proteins were refolded in the presence of hemin and urea, the refolded proteins showed peroxidase activity. Now, I injected the refolded proteins into mice to prepare monoclonal antibodies. In addition, I conducted the expression of morphine peroxidase which degrades morphine in opium poppy, and started to prepare its anbodies.
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