Exhaustive identification of mutations conferring drug resistance in influenza virus NA
Project/Area Number |
16K15151
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Environmental and hygienic pharmacy
|
Research Institution | University of Shizuoka |
Principal Investigator |
|
Co-Investigator(Renkei-kenkyūsha) |
IKEDA Kiyoshi 広島国際大学, 薬学部, 教授 (40168125)
|
Project Period (FY) |
2016-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | インフルエンザウイルス / 抗インフルエンザ薬 / 薬剤耐性 / シアリダーゼ / ノイラミニダーゼ / イメージング / 感染細胞 / 分離 / 単離 / 蛍光イメージング |
Outline of Final Research Achievements |
Influenza A and B viruses and their infected cells express an enzyme “sialidase”. We developed and commercialized a probe “BTP3-Neu5Ac” for fluorescence imaging of sialidase activity. We developed a novel technique for selective fluorescence cell imaging of drug-resistant virus-infected cells and for high-efficiency isolation of drug-resistant viruses, as application of BTP3-Neu5Ac. For the purpose of exhaustive identification of sialidase mutations conferring drug resistance, we tried viral sialidase expression in yeast but did not succeed. As an alternative way, we developed an easy and speedy method for detecting drug-resistant virus by use of BTP3-Neu5Ac. This method is expected to enable efficient detection of drug-resistant viruses and to contribute to progress of research on drug resistance mechanism.
|
Report
(3 results)
Research Products
(105 results)
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[Journal Article] Down-regulation of glutamate release from hippocampal neurons by sialidase2018
Author(s)
Akira Minami, Ami Ishii, Sumika Shimba, Takahiro Kano, Eri Fujioka, Saki Sai, Nagisa Oshio, Sayaka Ishibashi, Tadanobu Takahashi, Yuuki Kurebayashi, Hiroaki Kanazawa, Nobuhiro Yuki, Tadamune Otsubo, Kiyoshi Ikeda and Takashi Suzuki
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Journal Title
J. Biochem.
Volume: 163
Issue: 4
Pages: 273-280
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Rapid regulation of sialidase activity in response to neural activity and sialic acid removal during memory processing in rat hippocampus.2017
Author(s)
Akira Minami, Yuko Meguro, Sayaka Ishibashi, Ami Ishii, Mako Shiratori, Saki Sai, Yuuki Horii, Hirotaka Shimizu, Hokuto Fukumoto, Sumika Shimba, Risa Taguchi, Tadanobu Takahashi, Tadamune Otsubo, Kiyoshi Ikeda and Takashi Suzuki
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Journal Title
J. Biol. Chem.
Volume: 292
Pages: 5645-5654
Related Report
Peer Reviewed
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[Journal Article] Rapid regulation of sialidase activity in response to neural activity and sialic acid removal during memory processing in rat hippocampus.2017
Author(s)
Akira Minami, Yuko Meguro, Sayaka Ishibashi, Ami Ishii, Mako Shiratori, Saki Sai, Yuuki Horii, Hirotaka Shimizu, Hokuto Fukumoto, Sumika Shimba, Risa Taguchi, Tadanobu Takahashi, Tadamune Otsubo, Kiyoshi Ikeda and Takashi Suzuki.
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Journal Title
J. Biol. Chem.
Volume: 292
Issue: 14
Pages: 5645-5654
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Substrate Specificity of Equine and Human Influenza A Virus Sialidase to Molecular Species of Sialic Acid2016
Author(s)
Tadanobu Takahashi, Saori Unuma, Sawako Kawagishi, Yuuki Kurebayashi, Maiko Takano, Hiroki Yoshino, Akira Minami, Takashi Yamanaka, Tadamune Otsubo, Kiyoshi Ikeda, Takashi Suzuki.
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Journal Title
Biological and Pharmaceutical Bulletin
Volume: 39
Issue: 10
Pages: 1728-1733
DOI
NAID
ISSN
0918-6158, 1347-5215
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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