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Understanding of the purinergic signaling using spider toxins and insecticides

Research Project

Project/Area Number 16K15180
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General physiology
Research InstitutionKagawa University (2018)
Osaka University (2016-2017)

Principal Investigator

Fujiwara Yuichiro  香川大学, 医学部, 教授 (20532980)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsion channel / イオンチャネル / ATP受容体 / 生理学 / 薬理学
Outline of Final Research Achievements

Purinergic ATP receptor channels (P2X) are responsible for maintaining various physiological functions such as pain sensation and transmission, dilation of blood vessels and inflammatory reaction. These physiological functions are exhibited by membrane excitation and Ca2+ dependent cellular reactions through P2X. In this study we explored the molecular basis of ion selectivity and its physiological roles using blockers and activators of P2X.

Academic Significance and Societal Importance of the Research Achievements

ATP受容体のP2Xは、痛みの受容と伝達、血管の拡張、炎症反応など様々な生理機能に関係している。これら生理機能はP2Xから流入する陽イオンによる膜の興奮やCa2+による細胞内応答により発揮される。本研究により、P2Xのイオンの透過性の分子基盤とその生理機能が明らかになったことにより、将来的には、新しいタイプの鎮痛薬や抗高血圧薬への開発が期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (18 results)

All 2019 2018 2017 2016 Other

All Journal Article (8 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 8 results,  Open Access: 6 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Int'l Joint Research: 3 results,  Invited: 6 results) Remarks (1 results)

  • [Journal Article] Structural Basis for the Membrane Association of Ankyrin2018

    • Author(s)
      藤原 祐一郎, 近藤 寛子, 城田 松之, 木下 賢吾
    • Journal Title

      Seibutsu Butsuri

      Volume: 58 Issue: 3 Pages: 152-154

    • DOI

      10.2142/biophys.58.152

    • NAID

      130007378614

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Temperature-Sensitive Gating of Voltage-Gated H+ Channels2017

    • Author(s)
      Fujiwara Y.
    • Journal Title

      Journal of Physiological Sciences, Supplement1

      Volume: 67

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] A study of dynamics of palmitoylated Ankyrin-G around a lipid bilayer by coarse-grained simulations2017

    • Author(s)
      Kondo XH, Fujiwara Y., Shirota M., Kinoshita K.
    • Journal Title

      Journal of Physiological Sciences, Supplement1

      Volume: 67

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structural insights into the nucleotide base specificity of P2X receptors.2017

    • Author(s)
      Kasuya G, Fujiwara Y, Tsukamoto H, Morinaga S, Ryu S, Touhara K, Ishitani R, Furutani Y, Hattori M, Nureki O.
    • Journal Title

      Sci Rep.

      Volume: 7 Issue: 1 Pages: 45208-45208

    • DOI

      10.1038/srep45208

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Conductance of P2X4 receptor is determined by conformational equilibrium in the transmembrane region2016

    • Author(s)
      Minato Y, Suzuki S , Hara T, Kofuku Y, Kasuya G, Fujiwara Y, Igarashi S, Suzuki E, Nureki O, Hattori M, Ueda T, Shimada I
    • Journal Title

      Proc. Natl. Acad. Sci.

      Volume: 発行中 Issue: 17 Pages: 4741-4746

    • DOI

      10.1073/pnas.1600519113

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Comparison between mouse and sea urchin orthologs of voltage-gated proton channel suggests role of S3 segment in activation gating2016

    • Author(s)
      Sakata S, Miyawaki N, McCormack TJ, Arima H, Kawanabe A, Ozkucur N, Kurokawa T, Jinno Y, Fujiwara Y, Okamura Y
    • Journal Title

      Biochem. Biophys. Acta.

      Volume: 1858 Issue: 12 Pages: 2972-2983

    • DOI

      10.1016/j.bbamem.2016.09.008

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Crystal structures of the TRIC trimeric intracellular cation channel orthologues.2016

    • Author(s)
      Kasuya G, Hiraizumi M, Maturana AD, Kumazaki K, Fujiwara Y, Liu K, Nakada-Nakura Y, Iwata S, Tsukada K, Komori T, Uemura S, Goto Y, Nakane T, Takemoto M, Kato HE, Yamashita K, Wada M, Ito K, Ishitani R, Hattori M, Nureki O.
    • Journal Title

      Cell Res.

      Volume: 26 Issue: 12 Pages: 1288-1301

    • DOI

      10.1038/cr.2016.140

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Structural basis for the membrane association of ankyrinG via palmitoylation2016

    • Author(s)
      Fujiwara Y, Kondo H, Shirota M, Kobayashi M, Takeshita K, Nakagawa A, Okamura Y, Kinoshita K
    • Journal Title

      Sci. Rep.

      Volume: 6 Issue: 1 Pages: 23981-23981

    • DOI

      10.1038/srep23981

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] What is the pH-gradient Sensing in the Voltage- Gated H+ Channel?2019

    • Author(s)
      藤原祐一郎
    • Organizer
      第9回アジアオセアニア生理学会(第96回日本生理学会合同大会)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Toward the understanding of hexose specificity of Na+ D-glucose cotransporters SGLT1 and SGLT22019

    • Author(s)
      神鳥和代、藤原祐一郎
    • Organizer
      第9回アジアオセアニア生理学会(第96回日本生理学会合同大会)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ion Permeation of Voltage Sensor and its Foundation Structure2019

    • Author(s)
      片木絢子、藤原祐一郎
    • Organizer
      第9回アジアオセアニア生理学会(第96回日本生理学会合同大会)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 電位依存性H+チャネルの電気的・化学的勾配センシング機構2018

    • Author(s)
      藤原祐一郎
    • Organizer
      蛋白研研究会「構造情報に基づいた膜イオン輸送タンパク質の 生理機能の解明に向けて」
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Approaches to structural dynamics of the voltage-gated H+ channel gating.2018

    • Author(s)
      藤原祐一郎
    • Organizer
      第95回日本生理学会大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 電気生理で蛋白質を解析する2017

    • Author(s)
      藤原祐一郎
    • Organizer
      第66回NCVC研究者交流会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 電位依存性プロトンチャネルの温度感受性ゲーティング2017

    • Author(s)
      藤原祐一郎
    • Organizer
      第94回日本生理学会大会
    • Place of Presentation
      浜松
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 粗視化シミュレーションによるアンキリンGの細胞膜周辺での動態の解析2017

    • Author(s)
      近藤寛子、藤原祐一郎、城田松之、木下 賢吾
    • Organizer
      第94回日本生理学会大会
    • Place of Presentation
      浜松
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 電位依存性H+チャネルのゲーティングとその構造基盤2017

    • Author(s)
      藤原祐一郎
    • Organizer
      Advanced Biological Chemistry Seminar 2017
    • Place of Presentation
      京都
    • Related Report
      2016 Research-status Report
    • Invited
  • [Remarks] 温度生物学トピックス「TRPV1の熱活性はポアドメインに備わっている。」

    • URL

      http://www.nips.ac.jp/thermalbio/report/report2018_01.html

    • Related Report
      2018 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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