Molecular mechanism for highly sensitive response to peripheral hormones in hypothalamic neurons and homeostatic role
| Project/Area Number |
16K15182
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | Jichi Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MASANORI Nakata 自治医科大学, 医学部, 准教授 (10305120)
TATSUYA Dezaki 自治医科大学, 医学部, 准教授 (90337329)
YUSAKU Iwasaki 自治医科大学, 医学部, 講師 (60528420)
SHIGETOMO Suyama 自治医科大学, 医学部, 助教 (80528414)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | インスリン / 視床下部 / 室傍核 / 神経内分泌 / オキシトシン神経 / PDK１ / PDK1 / 高感受性 / インスリン感受性 / 細胞情報伝達 / 弓状核 / 視交叉上核 |
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| Outline of Final Research Achievements |
Peripheral injection of insulin induced c-Fos in hypothalamic paraventricular nucleus (PVN) particularly in large-sized oxytocin neurons, and increased plasma oxytocin levels in mice. Very low levels of nsulin at picomolar concentrations increased cytosolic free calcium in single neurons isolated from PVN mostly in large-sized neuroendocrine oxytocin neurons. In oxytocin neuron-specific PDK1 knockdown mice via Cre-LoxP system, picomolar insulin failed to activate neuroendocrine oxytocin neurons and peripheral insulin-induced rises in plasma oxytocin disappeared.
These results indicate that large-sized neuroendocrine oxytocin neurons in PVN respond to very low levels of insulin via mechanisms involving PDK1.
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] Endogenous α2A-adrenoceptor-operated sympathoadrenergic tones attenuate insulin secretion via cAMP/TRPM2 signaling.2017
Author(s)
Ito K, Dezaki K, Yoshida M, Yamada H, Miura R, Rita RS, Ookawara S, Tabei K, Kawakami M, Hara K, Morishita Y, Yada T, Kakei M
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Journal Title
Diabetes
Volume: 66(3)
Issue: 3
Pages: 699-709
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Fibroblast growth factor 21, assisted by elevated glucose, activates paraventricular nucleus NUCB2/Nesfatin-1 neurons to produce satiety under fed states.2017
Author(s)
Santoso P, Nakata M, Shiizaki K, Boyang Z, Parmila K, Otgon-Uul Z, Hashimoto K, Satoh T, Mori M, Kuro-O M, Yada T
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Journal Title
Sci Rep
Volume: 印刷中
Issue: 1
Pages: 45819-45819
DOI
Related Report
Peer Reviewed / Open Access
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