| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Outline of Final Research Achievements |
A piece of specimen was cultured after dissecting out from apple fruit, which had been naturally cultivated under a no pesticide-, no fertilizer-condition. Although the specimen had been disinfected from the outside when cultured just after the dissection, fungal spores appeared possibly from the inside of the specimen. Incidentally, an apple fruit polyphenol in exocarp inhibits bacterial sugar metabolism. Bacterial metabolites in turn suppress a sugar transporter of fungi. As such, I hypothesized that apple fruit could well suppress fungi indirectly through regulation of the bacteria with the polyphenol. To test this, I planned to explore the interaction of these microorganisms with fluorescent sugar derivatives. Preliminary experiments, however, revealed that the sugar uptake was too heterogeneous to see with a conventional method, making us to develop a new three-dimensional, multiple angle imaging method. We hope this method will be a tool to elucidate the complex interaction.
|
