A study on antifungal effect of apple fruit polyphenol
| Project/Area Number |
16K15186
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental physiology(including physical medicine and nutritional physiology)
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| Research Institution | Hirosaki University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 真菌 / ポリフェノール / 果実 / 蛍光グルコース / 栄養生理学 / イメージング |
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| Outline of Final Research Achievements |
A piece of specimen was cultured after dissecting out from apple fruit, which had been naturally cultivated under a no pesticide-, no fertilizer-condition. Although the specimen had been disinfected from the outside when cultured just after the dissection, fungal spores appeared possibly from the inside of the specimen. Incidentally, an apple fruit polyphenol in exocarp inhibits bacterial sugar metabolism. Bacterial metabolites in turn suppress a sugar transporter of fungi. As such, I hypothesized that apple fruit could well suppress fungi indirectly through regulation of the bacteria with the polyphenol. To test this, I planned to explore the interaction of these microorganisms with fluorescent sugar derivatives. Preliminary experiments, however, revealed that the sugar uptake was too heterogeneous to see with a conventional method, making us to develop a new three-dimensional, multiple angle imaging method. We hope this method will be a tool to elucidate the complex interaction.
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Report
(2 results)
Research Products
(11 results)
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[Journal Article] Unknown biological effects of l-glucose, ALA, and PUFA2017
Author(s)
Yamada, K., Sato, D., Nakamura, T., Amano, H., and Morimoto, Y.
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Journal Title
The Journal of Physiological Sciences
Volume: 67
Pages: 539-548
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Sugar metabolism imaging in three dimensionally cultured Ewing sarcoma cells using fluorescent glucose derivative2018
Author(s)
Ohshika, S., Sasaki, A., Ogawa, T., Yanagisawa, M., Ishibashi, Y., Yamada, K.
Organizer
Orthopaedic Research Society (ORS) 2018 Annual Meeting
Related Report
Int'l Joint Research
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