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Suppressor screening for sleep in mice

Research Project

Project/Area Number 16K15187
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Environmental physiology(including physical medicine and nutritional physiology)
Research InstitutionUniversity of Tsukuba

Principal Investigator

FUNATO Hiromasa  筑波大学, 国際統合睡眠医科学研究機構, 教授(WPI-IIIS) (90363118)

Co-Investigator(Renkei-kenkyūsha) YANAGISAWA MASASHI  筑波大学, 国際統合睡眠医科学研究機構, 機構長教授 (20202369)
MIYOSHI CHIKA  筑波大学, 国際統合睡眠医科学研究機構, 助教 (60613437)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords睡眠覚醒 / フォワード・ジェネティクス / リン酸化酵素 / 細胞内シグナル伝達系 / サプレッサースクリーニング / 睡眠
Outline of Final Research Achievements

The principal investigator conducted a suppressor screening to identify molecules involved in sleep regulation through the SIK3 pathway. We performed whole exosome sequencing to examine the frequency of random point mutations due to ethylnitrosourea dosage. We used SIK3 gene mutant mice of the C57BL/6N strain to induce random mutations and made them crossed with wild-type C57BL/6J females to obtain F1 mice. We examined sleep/wake behavior based on EEG/EMG recording of the F1 generation mice. Since there are many polymorphisms even in the same C57BL/6J or C57BL/6N strains from multiple breeders, we sequenced SNP candidates and created a full SNP panel that is necessary for a linkage analysis. I have been running a suppressor screening for sleep in mice.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (16 results)

All 2018 2017 2016

All Journal Article (8 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 8 results,  Open Access: 7 results,  Acknowledgement Compliant: 4 results) Presentation (8 results) (of which Int'l Joint Research: 2 results,  Invited: 6 results)

  • [Journal Article] Immunolocalization of muscarinic M1 receptor in the rat medial prefrontal cortex2018

    • Author(s)
      Oda Satoko、Tsuneoka Yousuke、Yoshida Sachine、Adachi-Akahane Satomi、Ito Masanori、Kuroda Masaru、Funato Hiromasa
    • Journal Title

      Journal of Comparative Neurology

      Volume: 526 Issue: 8 Pages: 1329-1350

    • DOI

      10.1002/cne.24409

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sleep/Wake Behaviors in Mice During Pregnancy and Pregnancy-Associated Hypertensive Mice2017

    • Author(s)
      Komiya Haruna、Miyoshi Chika、Iwasaki Kanako、Hotta-Hirashima Noriko、Ikkyu Aya、Kanno Satomi、Honda Takato、Gosho Masahiko、Hamada Hiromi、Satoh Toyomi、Fukamizu Akiyoshi、Funato Hiromasa、Yanagisawa Masashi
    • Journal Title

      Sleep

      Volume: 41 Issue: 3 Pages: 1-1

    • DOI

      10.1093/sleep/zsx209

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Neurotransmitters and neuropeptides in gonadal steroid receptor-expressing cells in medial preoptic area subregions of the male mouse2017

    • Author(s)
      Yousuke Tsuneoka, Sachine Yoshida, Kenkichi Takase, Satoko Oda, Masaru Kuroda, Hiromasa Funato
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 1-16

    • DOI

      10.1038/s41598-017-10213-4

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Moxd1 Is a Marker for Sexual Dimorphism in the Medial Preoptic Area, Bed Nucleus of the Stria Terminalis and Medial Amygdala2017

    • Author(s)
      Tsuneoka Y and Funato H et al.
    • Journal Title

      Front Neuroanat

      Volume: 11 Pages: 26-26

    • DOI

      10.3389/fnana.2017.00026

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Forward-genetics analysis of sleep in randomly mutagenized mice2016

    • Author(s)
      Funato H and Yanagisawa M et al.
    • Journal Title

      Nature

      Volume: 539(7629) Issue: 46 Pages: 378-383

    • DOI

      10.1038/nature20142

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Identification of mutations through dominant screening for obesity using C57BL/6 substrains2016

    • Author(s)
      Hossain MS., Funato H, and Yanagisawa M et al.
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 32453-32453

    • DOI

      10.1038/srep32453

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] High-fat diet feeding alters olfactory-, social-, and reward-related behaviors of mice independent of obesity.2016

    • Author(s)
      Takase K, Tsuneoka Y, Oda S, Kuroda M, Funato H
    • Journal Title

      Obesity

      Volume: 4 Issue: 4 Pages: 886-894

    • DOI

      10.1002/oby.21441

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Sleep as a biological problem: an overview of frontiers in sleep research2016

    • Author(s)
      Kanda T, Tsujino N, Kuramoto E, Koyama Y, Susaki EA, Chikahisa S, Funato H
    • Journal Title

      J Physiol Sci

      Volume: 66 Issue: 1 Pages: 1-13

    • DOI

      10.1007/s12576-015-0414-3

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 視索前野の性差とその発生2018

    • Author(s)
      船戸弘正
    • Organizer
      第123回日本解剖学会総会・全国学術集会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Forward genetic analysis of sleep in mice2018

    • Author(s)
      Hiromasa Funato
    • Organizer
      Gordon Research Conference "Sleep Regulation and Function"
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Forward genetic analysis of sleep in mice2018

    • Author(s)
      Hiromasa Funato, Masashi Yanagisawa
    • Organizer
      Keystone symposia "State of the Brain"
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] フォワード・ジェネティクスによる睡眠制御遺伝子の同定2017

    • Author(s)
      船戸弘正
    • Organizer
      第122回日本解剖学会総会学術集会
    • Place of Presentation
      長崎大学(長崎市)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] オレキシン受容体とSIK3による睡眠制御2017

    • Author(s)
      船戸弘正
    • Organizer
      第36回日本社会精神医学会
    • Place of Presentation
      大田区産業プラザ(東京都大田区)
    • Year and Date
      2017-03-04
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] マウスを用いた睡眠のフォワード・ジェネティクス研究2017

    • Author(s)
      船戸弘正
    • Organizer
      第24回日本時間生物学会学術大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 眠りの謎に挑む-睡眠科学の最前線2017

    • Author(s)
      船戸弘正
    • Organizer
      第39回茨城医学会総会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] フォワード・ジェネティクスによる過眠と肥満をもたらす遺伝子変異の同定2017

    • Author(s)
      船戸弘正
    • Organizer
      第23回日本行動医学会学術総会
    • Place of Presentation
      沖縄科学技術大学院大学(恩納村)
    • Related Report
      2016 Research-status Report
    • Invited

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Published: 2016-04-21   Modified: 2019-03-29  

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